Expression analysis in response to drought stress in soybean: Shedding light on the regulation of metabolic pathway genes.
Expression analysis in response to drought stress in soybean: Shedding light on the regulation of metabolic pathway genes.
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DOI：
10.1590/s1415-47572012000200004
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发表时间：
2012-06
影响因子：
2.1
通讯作者：
Alves-Ferreira M
中科院分区：
文献类型：
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作者：
Guimarães-Dias F;Neves-Borges AC;Viana AA;Mesquita RO;Romano E;de Fátima Grossi-de-Sá M;Nepomuceno AL;Loureiro ME;Alves-Ferreira M
野生型拟南芥植物的代谢组学分析，在控制和干旱胁迫条件下，揭示了几种代谢途径，诱导水分亏缺。干旱胁迫的代谢反应也与阿坝依赖和独立的途径，使在这个模式植物的分子机制更好地理解。本研究旨在通过计算机模拟技术和实时荧光定量PCR技术相结合的方法，对大豆水分亏缺相关代谢途径基因进行鉴定。利用Genevestigator软件分析了干旱胁迫条件下拟南芥基因的数字表达模式。选择在干旱胁迫下表现出强诱导的基因，并将其用作诱饵以鉴定大豆基因组中的直系同源物。这使我们能够选择属于38个不同代谢途径的79个拟南芥基因的354个推定的大豆直系同源基因。在GENOSOJA项目中可用的消减文库中验证了所选基因的表达模式。随后，选择来自不同代谢途径的13个基因，通过qPCR实验进行验证。六个基因的表达进行了验证，在植物经历干旱胁迫在基于盆和水培栽培系统。结果表明，干旱胁迫的代谢反应在拟南芥和大豆植物中是保守的。
Metabolomics analysis of wild type Arabidopsis thaliana plants, under control and drought stress conditions revealed several metabolic pathways that are induced under water deficit. The metabolic response to drought stress is also associated with ABA dependent and independent pathways, allowing a better understanding of the molecular mechanisms in this model plant. Through combining an in silico approach and gene expression analysis by quantitative real-time PCR, the present work aims at identifying genes of soybean metabolic pathways potentially associated with water deficit. Digital expression patterns of Arabidopsis genes, which were selected based on the basis of literature reports, were evaluated under drought stress condition by Genevestigator. Genes that showed strong induction under drought stress were selected and used as bait to identify orthologs in the soybean genome. This allowed us to select 354 genes of putative soybean orthologs of 79 Arabidopsis genes belonging to 38 distinct metabolic pathways. The expression pattern of the selected genes was verified in the subtractive libraries available in the GENOSOJA project. Subsequently, 13 genes from different metabolic pathways were selected for validation by qPCR experiments. The expression of six genes was validated in plants undergoing drought stress in both pot-based and hydroponic cultivation systems. The results suggest that the metabolic response to drought stress is conserved in Arabidopsis and soybean plants.