A standardized procedure for quantitation of CD11b on polymorphonuclear neutrophil by flow cytometry: potential application in infectious diseases
A standardized procedure for quantitation of CD11b on polymorphonuclear neutrophil by flow cytometry: potential application in infectious diseases
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DOI：
10.1111/j.1365-2257.2004.00599.x
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发表时间：
2004-06-01
期刊：
影响因子：
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通讯作者：
Lecompte, T
中科院分区：
文献类型：
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作者：
Latger-Cannard, V;Besson, I;Lecompte, T
在多形核白细胞（PMN）细胞活化过程中，表面标志物CD11b的上调已得到证实。CD11b过度表达通常与炎症相关，被认为是感染的早期标志物。然而.由于缺乏标准化的检测方法和分析前设置的可变性导致PMN人为激活，因此损害了对该标志物的兴趣。在本研究中，直接在全血中进行的标准化定量流式细胞术测定已被用于确定PMN细胞上的CD11b表达。结果表明，如果使用包括特定校准品、试剂和方案的校准系统，定量流式细胞术可以在实验室之间提供一致的CD11b密度值。这种方法使我们能够证明感染患者的CD11b表达上调。这种定量是一种标准化的，在临床情况下可能有用的方法，意味着定量CD11b表达的变化。
An up-regulation of the surface marker CD11b has been demonstrated during polymorphonuclear (PMN) cell activation. CD11b over-expression is often associated with inflammation and is considered as an early marker of infection. However. the absence of standardized assay and the variability of preanalytical settings leading to PMN artifactual activation have compromised the interest of this marker. In the present study a standardized quantitative flow cytometry assay directly performed in whole blood has been used to determine CD11b expression on PMN cells. The results indicate that quantitative flow cytometry can provide consistent CD11b density values between laboratories provided that a calibration system is used including specific calibrators, reagents and protocols. This method allowed us to evidence an up-regulation of CD11b expression for infected patients. This quantitation is a standardized and potentially useful method in clinical situations implying quantitative CD11b expression variations.