Basal chloride currents in murine airway epithelial cells: modulation by CFTR.
Basal chloride currents in murine airway epithelial cells: modulation by CFTR.
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小鼠气道上皮细胞中的基础氯电流：CFTR 的调节。
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
B. Argent
中科院分区：
文献类型：
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作者：
R. Tarran;M. Gray;M. J. Evans;W. Colledge;R. Ratcliff;B. Argent
我们从野生型和囊性纤维化（CF）空小鼠的鼻上皮分离纤毛呼吸细胞，并使用膜片钳技术研究其基础电导。未受刺激的细胞的电流钳实验表明存在的K+和Cl-电导，并在一定条件下，一个小的Na+电导。电压钳实验显示三个不同的Cl-电导。I tv-indep不依赖于时间和电压，具有线性电流-电压（I-V）图; I v-act在电位大于± 50 mV时抑制激活，给出S形I-V图; I hyp-act被超极化电位激活，具有向内整流的I-V图。电流密度的大小顺序为I hyp-act = I v-act = I tv-indep。这些电导率的Cl-与N-甲基-D-葡糖胺阳离子渗透率比值在2.8和10.3之间，不受他莫昔芬、氟芬那酯、格列本脲、DIDS和5-硝基-2-（3-苯丙氨基）苯甲酸的影响，但受Zn 2+和Gd 3+的抑制。I tv-indep和I v-act以相等的密度和频率存在于野生型和CF细胞中。然而，与野生型细胞的26%相比，仅在3%的CF细胞中检测到I hyp-act，这表明这种传导可能受到囊性纤维化跨膜传导调节因子（CFTR）的调节。
We have isolated ciliated respiratory cells from the nasal epithelium of wild-type and cystic fibrosis (CF) null mice and used the patch-clamp technique to investigate their basal conductances. Current-clamp experiments on unstimulated cells indicated the presence of K+ and Cl- conductances and, under certain conditions, a small Na+conductance. Voltage-clamp experiments revealed three distinct Cl- conductances. I tv-indep was time and voltage independent with a linear current-voltage ( I- V) plot; I v-actexhibited activation at potentials greater than ±50 mV, giving an S-shaped I- Vplot; and I hyp-act was activated by hyperpolarizing potentials and had an inwardly rectified I- Vplot. The current density sequence was I hyp-act = I v-act ≫ I tv-indep. These conductances had Cl--to- N-methyl-d-glucamine cation permeability ratios of between 2.8 and 10.3 and were unaffected by tamoxifen, flufenamate, glibenclamide, DIDS, and 5-nitro-2-(3-phenylpropylamino) benzoic acid but were inhibited by Zn2+ and Gd3+. I tv-indep and I v-act were present in wild-type and CF cells at equal density and frequency. However, I hyp-actwas detected in only 3% of CF cells compared with 26% of wild-type cells, suggesting that this conductance may be modulated by cystic fibrosis transmembrane conductance regulator (CFTR).
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影响因子：
3.5
作者：
CID, LP;MONTROSERAFIZADEH, C;CUTTING, GR
通讯作者：
CUTTING, GR
影响因子：
56.9
作者：
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通讯作者：
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收缩激活非选择性电导：Walker 基序蛋白和 PKC 的参与。
DOI：
10.1152/ajpcell.1996.270.1.c179
发表时间：
1996
期刊：
The American journal of physiology.
影响因子：
--
作者：
Nelson,DJ;Tien,XY;Xie,W;Brasitus,TA;Kaetzel,MA;Dedman,JR
通讯作者：
Dedman,JR
影响因子：
--
作者：
WILLUMSEN, NJ;DAVIS, CW;BOUCHER, RC
通讯作者：
BOUCHER, RC
人结肠细胞系 T84 中的体积敏感氯离子电导。
DOI：
10.1152/ajpcell.1989.256.6.c1111
发表时间：
1989
期刊：
The American journal of physiology
影响因子：
--
作者：
Worrell,RT;Butt,AG;Cliff,WH;Frizzell,RA
通讯作者：
Frizzell,RA