Analysis of the effect of nutritional factors on OTA and OTB biosynthesis and polyketide synthase gene expression in Aspergillus ochraceus
Analysis of the effect of nutritional factors on OTA and OTB biosynthesis and polyketide synthase gene expression in Aspergillus ochraceus
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DOI：
10.1016/j.ijfoodmicro.2009.07.014
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发表时间：
2009-09-30
影响因子：
5.4
通讯作者：
Dobson, Alan D. W.
中科院分区：
文献类型：
--
作者：
Abbas, Abdelhamid;Valez, Heriberto;Dobson, Alan D. W.
研究了多种营养生物因子对赭曲霉产OTA和OTB生物合成的影响。不同的碳源，包括葡萄糖、蔗糖、麦芽糖、半乳糖、木糖和甘油，在OTA允许的PDY培养基上生长时，似乎抑制了OTA的产生。相反，乳糖似乎诱导OTA的产生，在OTA限制性PDC培养基中添加乳糖和半乳糖，导致OTA水平显著增加。在MCB和PDY培养基中添加乳糖可显著提高OTB产量。在MCB中添加蔗糖和半乳糖也有类似的效果，但不太明显。不同的氮源也会影响OTA的产生：氯化铵显著降低OTA的产生，而尿素等有机氮源以及苯丙氨酸、赖氨酸、谷氨酰胺和脯氨酸等氨基酸诱导OTA的产生。在这些条件下诱导otapks Ao基因的表达与在相同实验条件下产生的OTA水平有很好的相关性，这表明观察到的影响似乎至少部分地在基因转录水平上受到调节。然而，虽然这些以营养为基础的生物因素似乎也影响到赭曲霉菌产生的OTB水平，但这似乎是以一种独立于otapks Ao基因表达的方式进行调节的。(C)2009爱思唯尔B.V.保留所有权利。
The effect of a wide variety of nutritional based biotic factors on the production of both OTA and OTB biosynthesis in A. ochraceus was assessed. Different carbon sources including glucose, sucrose, maltose, galactose, xylose and glycerol appear to repress OTA production when the fungus is grown in OTA permissive PDY medium. In contrast lactose appears to induce OTA production, with the addition of lactose and galactose to the OTA restrictive PDC medium resulting in marked increases in OTA levels. The addition of lactose to MCB and PDY media considerably increases OTB production. The addition of both sucrose and galactose to MCB has similar yet less marked effects. Different nitrogen sources also affect: OTA production with ammonium chloride significantly reducing OTA production, while organic nitrogen sources such as urea and amino acids including phenylalanine, lysine, glutamine and proline induce OTA production. The induction of otapksAo gene expression under these conditions correlates well with the levels of OTA produced under the same experimental conditions, suggesting that the observed effects appear to be modulated, at least in part, at the level of gene transcription. However while the levels of OTB produced in A. ochraceus also appear to be influenced by these nutritional based biotic factors, this appears to be regulated in a manner which is independent of otapksAo gene expression. (C) 2009 Elsevier B.V. All rights reserved.