XYLANASE-ACTIVITY, CM-CELLULASE-ACTIVITY AND INVERTASE ACTIVITY IN SOIL - AN IMPROVED METHOD
XYLANASE-ACTIVITY, CM-CELLULASE-ACTIVITY AND INVERTASE ACTIVITY IN SOIL - AN IMPROVED METHOD
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DOI：
10.1016/0038-0717(90)90187-5
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发表时间：
1990-01-01
影响因子：
9.7
通讯作者：
VONMERSI, W
中科院分区：
文献类型：
--
作者：
SCHINNER, F;VONMERSI, W
我们提出了一种定量测定土壤中木聚糖酶、CM纤维素酶或转化酶活性的方法。有可能提供一种简单、灵敏(2.8微克葡萄糖毫升-1分析混合物)和可重复性地测定农业和林业用土壤的酶电位。活性的测定通过底物(二甲烷、CM-纤维素或蔗糖)进行，反应在pH 5.5和50℃下进行。与六氰化铁试剂反应后，用比色法测定还原糖。进一步的研究证实了土壤纤维素酶的高度稳定性，并进一步证实了该酶和木聚糖酶与7种不同土壤腐殖质含量的高度相关性。
We present a method for the quantification of xylanase-, CM-cellulase- or invertase activities of soils. It is possible to offer a simple, sensitive (2.8 .mu.g glucose ml-1 assay mixture) and reproducible determination of the enzyme potentials of soils used by agriculture and forestry. The determination of the activity is carried out via the substrate (xylane, CM-cellulose or sucrose), the reaction is performed at pH 5.5 and 50.degree. C. The reducing sugars are measured colorimetrically after reaction with a potassium ferric hexa-cyanide reagent. Additional investigations confirm the high stability of the soil enzyme cellulase, and further the high correlation of this enzyme, and of the xylanase, with the humus content of 7 different soils.