Electrospun core-shell structured nanofilm as a novel colon-specific delivery system for protein.
Electrospun core-shell structured nanofilm as a novel colon-specific delivery system for protein.
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DOI：
10.1016/j.carbpol.2017.03.082
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发表时间：
2017-08
影响因子：
11.2
通讯作者：
Peng Wen;Kun Feng;Huang Yang;Xiao Huang;Min-hua Zong;Wenyong Lou;Ning Li;Hong Wu
中科院分区：
文献类型：
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作者：
Peng Wen;Kun Feng;Huang Yang;Xiao Huang;Min-hua Zong;Wenyong Lou;Ning Li;Hong Wu
以牛血清白蛋白（BSA）为蛋白质模型，采用同轴静电纺丝技术制备了一种新型的核壳结构蛋白质结肠递送纳米膜。首先采用离子凝胶法制备了BSA壳聚糖纳米粒子，然后以海藻酸钠为壳层，BSA壳聚糖纳米粒子为芯层制备了同轴纳米膜。扫描电子显微镜分析表明，所获得的纳米膜表现出光滑的表面，并通过透射电子显微镜的辅助证明了核壳结构。包封后BSA的二级结构变化不大，约75%的BSA在模拟结肠液中释放。建立了BSA在不同模拟消化液中的释放动力学模型，结果表明BSA在结肠中的释放遵循复杂的机制。本研究表明，静电纺丝纳米膜是一种很有前途的生物活性蛋白结肠定位给药系统。
A novel core-shell structured nanofilm for the delivery of protein to the colon was developed by coaxial electrospinning using bovine serum albumin (BSA) as protein model. Firstly, the BSA-loaded chitosan nanoparticle was prepared by ionic gelation, and then the coaxial nanofilm was fabricated using alginate as shell layer and the BSA-loaded chitosan nanoparticle as core layer. Scanning electron microscopy analysis showed that the obtained nanofilm exhibited a smooth surface, and the core-shell structure was evidenced by the aid of transmission electron microscopy. There was little change in the secondary structure of encapsulated BSA and around 75% of BSA was released in the simulated colonic fluid. The corresponding kinetics models of BSA release in different simulated digestive fluids were built and the results revealed that the release of BSA in colon followed a complex mechanism. This study shows that electrospun nanofilm is a promising colon-specific delivery system for bioactive protein.