Activation of AMPK by berberine promotes adiponectin multimerization in 3T3-L1 adipocytes
Activation of AMPK by berberine promotes adiponectin multimerization in 3T3-L1 adipocytes
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DOI：
10.1016/j.febslet.2011.04.051
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发表时间：
2011-06-23
期刊：
影响因子：
3.5
通讯作者：
Li, Zhen
中科院分区：
文献类型：
--
作者：
Li, Yun;Wang, Pengcheng;Li, Zhen
脂联素在脂肪细胞中组装成三聚体(LMW)、六聚体(MMW)和高分子量(HMW)多聚体。HMW脂联素在代谢方面更为活跃，与外周胰岛素敏感性密切相关。在本研究中，我们报道了一种具有胰岛素增敏作用的异喹啉生物碱，它抑制了脂联素的表达，但促进了HMW脂联素的组装，并增加了HMW/总脂联素的比例。黄连素激活AMPK。敲除AMPKα1可消除黄连素的作用。AICAR激活AMPK还可增加HMW脂联素水平。我们的研究表明，黄连素激活AMPK促进了脂联素的多聚化。蛋白质相互作用结构概述：脂联素与脂联素在凝胶电泳法中以迁移的方式相互作用(查看交互作用1，2)脂联素与脂联素通过密度梯度以共聚体的方式相互作用(查看交互作用)(C)2011年欧洲生化学会联合会。爱思唯尔出版，版权所有。
Adiponectin is assembled into trimer (LMW), hexamer (MMW) and high-molecular-weight (HMW) multimer in adipocytes. The HMW adiponectin is more metabolically active and closely associated with peripheral insulin sensitivity. In this study, we reported that berberine, an isoquinoline alkaloid with insulin-sensitizing effect, inhibits the expression of adiponectin, but promotes the assembly of HMW adiponectin and increases the ratio of HMW to total adiponectin. Berberine activates AMPK. Knockdown of AMPK alpha 1 abolishes the effect of berberine. Activation of AMPK by AICAR also increases the level of HMW adiponectin. Our study suggested that activation of AMPK by berberine promotes adiponectin multimerization.Structured summary of protein interactions:Adiponectin physically interacts with Adiponectin by comigration in gel electrophoresis (View Interaction 1, 2)Adiponectin physically interacts with Adiponectin by cosedimentation through density gradient (View interaction)(C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.