Transcriptional profiling analysis ofOsDT11-mediated ABA-dependent signal pathway for drought tolerance in rice
Transcriptional profiling analysis ofOsDT11-mediated ABA-dependent signal pathway for drought tolerance in rice
复制标题
OsDT11介导的ABA依赖性水稻耐旱信号通路的转录谱分析
DOI：
10.1007/s11816-020-00637-2
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发表时间：
2020
影响因子：
2.4
通讯作者：
Ding Xinhua
中科院分区：
文献类型：
--
作者：
Zhao Man;Ju Yanhu;Zhao Bo;Li Xiaoming;Dai LIli;Qu Jicheng;Chu Zhaohui;Ding Xinhua
富含半胱氨酸的小肽（CRP）在植物对生物和非生物胁迫的应答中起着重要作用，OsDT 11编码一个88个氨基酸的CRP类蛋白，可增强水稻的耐旱性。而不会对其他农艺性状产生负面影响。然而，OsDT 11介导的耐旱性的分子机制尚不清楚。在此，我们进行了RNA-Seq分析，以比较干旱胁迫和对照（非干旱）条件下野生型（WT）和OsDT 11过表达（OE）水稻系之间的转录组谱。与非干旱条件相比，在干旱处理下，OE系和WT中分别鉴定了1570和1421个差异表达基因。基因本体分析表明，共同的OE和WT系的430个上调基因诱导的功能相关的响应缺水和脱落酸（阿坝）。在干旱胁迫下，这些基因中有一半以上在OE中的表达高于WT。在OE中，而不是在WT中，294个基因在干旱胁迫下特异性上调，并且在淀粉和蔗糖生物合成过程中功能丰富，并响应于胁迫。这表明OsDT 11不仅能激发植物对干旱胁迫的强烈响应，而且还能通过调节多种代谢过程来增强植物的耐旱性。基因表达谱分析表明，OsDT 11通过ABA依赖的信号通路介导干旱胁迫应答增强，从而赋予植物耐旱性。
Small cysteine-rich peptides (CRPs) are important in plant responses to biotic and abiotic stresses.OsDT11, encoding an 88 amino acid CRP-type protein, has been reported to enhance tolerance to drought in rice (Oryza sativaL.) without negatively affecting other agronomic traits. However, the molecular mechanisms ofOsDT11-mediated drought tolerance are still unclear. Here, we performed RNA-Seq analysis to compare the transcriptome profiles between wild-type (WT) andOsDT11-overexpressing (OE) rice lines under drought stress or under control (non-drought) conditions. A total of 1570 and 1421 differentially expressed genes were identified in the OE lines and the WT under drought treatment, respectively, compared to non-drought conditions. Gene ontology analysis indicated that the 430 up-regulated genes in common to both OE and the WT lines were induced for functions related to responses to water deprivation and to abscisic acid (ABA). More than half of these genes had higher expression in the OE than in the WT under drought stress. In the OE, but not in the WT, 294 genes were specifically up-regulated under drought stress and were functionally enriched in starch and sucrose biosynthetic processes and in response to stress. This implies thatOsDT11not only triggers strongly response to drought stress, but also alters several metabolic processes to enhance drought tolerance. Gene expression profiling suggests thatOsDT11confers drought tolerance by mediating an enhanced response to drought stress in an ABA-dependent signaling pathways.