STRUCTURAL DIVERSITY IN THE PLASMODIUM-FALCIPARUM MEROZOITE SURFACE ANTIGEN-2
STRUCTURAL DIVERSITY IN THE PLASMODIUM-FALCIPARUM MEROZOITE SURFACE ANTIGEN-2
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DOI：
10.1073/pnas.88.5.1751
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发表时间：
1991-03-01
影响因子：
11.1
通讯作者：
ANDERS, RF
中科院分区：
文献类型：
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作者：
SMYTHE, JA;COPPEL, RL;ANDERS, RF
与疟疾寄生虫恶性疟原虫裂殖子表面相关的抗原可以直接受到免疫攻击，因此是主要的候选疫苗。 我们以前已经表明，两个已知的裂殖子表面抗原（裂殖子表面抗原2; MSA-2）表现出相当大的序列和抗原多样性在不同的分离株。 MSA-2从三个分离株的序列揭示了一个中央域组成的重复序列的数量，长度和序列不同，侧翼依次由非重复的可变序列和保守的N-和C-末端结构域。 我们在这里报告的序列的进一步四个MSA-2等位基因，含有重复序列，相关的，但不相同。 MSA-2的7个等位基因可根据非重复序列分为两个不同的等位基因家族。 重复探针杂交研究表明，所有44个恶性疟原虫分离株检查含有重复区类似于已知的MSA-2序列中定义的。
Antigens associated with the surface of merozoites of the malaria parasite Plasmodium falciparum are directly accessible to immune attack and therefore are prime vaccine candidates. We have previously shown that one of the two known merozoite surface antigens (merozoite surface antigen 2; MSA-2) exhibits considerable sequence and antigenic diversity in different isolates. The sequences of MSA-2 from three isolates revealed a central domain composed of repeats that vary in number, length, and sequence, flanked in turn by nonrepetitive variable sequences and by conserved N-and C-terminal domains. We report here the sequences of a further four MSA-2 alleles, containing repetitive sequences that are related but not identical to each other. The seven alleles of MSA-2 can be divided into two distinct allele families on the basis of nonrepetitive sequences. Hybridization studies with repeat probes indicated that all of the 44 P. falciparum isolates examined contained repeat regions similar to those defined in known MSA-2 sequences.