XRCC2 and XRCC3, new human Rad51-family members, promote chromosome stability and protect against DNA cross-links and other damages
XRCC2 and XRCC3, new human Rad51-family members, promote chromosome stability and protect against DNA cross-links and other damages
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DOI：
10.1016/s1097-2765(00)80078-7
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发表时间：
1998-05-01
期刊：
影响因子：
16
通讯作者：
Thompson, LH
中科院分区：
文献类型：
--
作者：
Liu, N;Lamerdin, JE;Thompson, LH
表型相似的仓鼠突变体irs1和irs1SF表现出高度的自发染色体不稳定性和广谱突变原敏感性，包括对DNA交联剂的极端敏感性。人类XRCC2和XRCC3基因分别在功能上与irs1和irs1SF互补，此前已在体细胞杂交中定位。这些基因的表征和序列对位表明，XRCC2和XRCC3是一个新兴的rad51相关蛋白家族的成员，可能参与同源重组以维持染色体稳定性和修复DNA损伤。研究表明，XRCC3与HsRad51直接相互作用，并且与酵母中的Rad55和Rad57一样，在重组修复过程中可能与HsRad51合作。对irs1中XRCC2突变的分析表明，XRCC2的功能对培养的仓鼠细胞的生存能力不是必需的。
The phenotypically similar hamster mutants irs1 and irs1SF exhibit high spontaneous chromosome instability and broad-spectrum mutagen sensitivity, including extreme sensitivity to DNA cross-linking agents. The human XRCC2 and XRCC3 genes, which functionally complement irs1 and irs1SF, respectively, were previously mapped in somatic cell hybrids. Characterization of these genes and sequence alignments reveal that XRCC2 and XRCC3 are members of an emerging family of Rad51-related proteins that likely participate in homologous recombination to maintain chromosome stability and repair DNA damage. XRCC3 is shown to interact directly with HsRad51, and like Rad55 and Rad57 in yeast, may cooperate with HsRad51 during recombinational repair. Analysis of the XRCC2 mutation in irs1 implies that XRCC2's function is not essential for viability in cultured hamster cells.