EXPRESSION OF A TRANSFECTED HUMAN C-MYC ONCOGENE INHIBITS DIFFERENTIATION OF A MOUSE ERYTHROLEUKEMIA CELL-LINE
EXPRESSION OF A TRANSFECTED HUMAN C-MYC ONCOGENE INHIBITS DIFFERENTIATION OF A MOUSE ERYTHROLEUKEMIA CELL-LINE
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DOI：
10.1038/322748a0
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发表时间：
1986-08-21
期刊：
影响因子：
64.8
通讯作者：
SEGAL, S
中科院分区：
文献类型：
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作者：
DMITROVSKY, E;KUEHL, WM;SEGAL, S
HIV-病毒衍生的1小鼠红白血病（MEL）细胞系代表转化的早期红系前体细胞，其可以通过包括二甲基亚砜（DMSO）的多种试剂诱导分化为更成熟的红系细胞。在加入诱导剂后有12小时的潜伏期，此时80-90%的细胞变得不可逆地致力于分化程序，在永久停止复制之前经历几轮细胞分裂3，4。DMSO诱导后，c-myc 5，6和c-myb 6信使RNA的稳态水平出现双相下降。在c-mycmRNA表达的初始降低之后，随后的增加发生在细胞周期的Gl期，并且仅限于细胞周期的Gl期7。我们试图确定下调是否是化学诱导分化的必要步骤。本文报道的实验表明，在MEL细胞中表达转染的人c-mycgene抑制终末分化过程。
The Friend-virus-derived1mouse erythroleukaemia (MEL) cell lines represent transformed early erythroid precursors that can be induced to differentiate into more mature erythroid cells by a variety of agents including dimethyl sulphoxide (DMSO)2There is a latent period of 12 hours after inducer is added, when 80–90% of the cells become irreversibly committed to the differentiation programme, undergoing several rounds of cell division before permanently ceasing to replicate3,4. After DMSO induction, a biphasic decline in steady-state levels of c-myc5,6and c-myb6messenger RNAs occurs. Following the initial decrease in c-mycmRNA expression, the subsequent increase occurs in, and is restricted to, the Glphase of the cell cycle7. We sought to determine whether the down-regulation is a necessary step in chemically induced differentiation. Experiments reported here indicate that expression in MEL cells of a transf ected human c-mycgene inhibits the terminal differentiation process.