Species and age related differences in the type and distribution of influenza virus receptors in different tissues of chickens, ducks and turkeys.
Species and age related differences in the type and distribution of influenza virus receptors in different tissues of chickens, ducks and turkeys.
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DOI：
10.1186/1743-422x-7-5
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发表时间：
2010-01-12
期刊：
影响因子：
4.8
通讯作者：
Lee CW
中科院分区：
文献类型：
--
作者：
Pillai SP;Lee CW
我们对不同年龄组的鸡、鸭和火鸡的不同组织上的 α2,3 唾液酸 (SA)-半乳糖 (gal)（禽类）和 α2,6SA-gal（人型）受体的分布进行了最详细的研究之一。在气管上皮上，所有 3 种鸟类在 3 个不同年龄组中都表达了 α2,3SA-gal 受体的强阳性染色（80-90%）。此外，观察到较少量的α2,6SA-gal受体（30-90%），其分布随年龄和物种的变化略有差异。火鸡和鸭的小肠和大肠上皮显示 α2,6SA-gal 受体的染色可忽略不计，而大肠始终显示 40-70% 的 α2,3SA-gal 受体阳性染色。相比之下，在鸡小肠和大肠上皮上观察到更多的α2,3SA-gal (50-80%) 和α2,6SA-gal (20-50%) 受体染色。这 3 种鸟类的肾脏和食道切片也表达了鸟类和人类类型的受体。在检查的其他组织中，大脑、胸肌、法氏囊、脾脏、盲肠扁桃体和输卵管、人类类型受体不存在。尽管不同的病毒和受体成分可能在成功的病毒复制和传播中发挥作用，但了解家禽不同组织中的受体类型和分布可能是了解促进成功流感病毒感染的宿主因素的良好初步工具。
We undertook one of the most detailed studies on the distribution of α2,3 sialic acid (SA)-galactose (gal) (avian type) and α2,6SA-gal (human type) receptors on different tissues of chickens, ducks and turkeys of varying age groups. On the tracheal epithelium, all 3 bird species expressed strong positive staining (80-90%) for α2,3SA-gal receptors in the 3 different age groups. In addition, a lesser amount of α2,6SA-gal receptors (30-90%) were observed with slight differences in distribution with age and species. The epithelium of the small and large intestine of turkeys and ducks showed negligible staining for α2,6SA-gal receptors whereas the large intestine consistently showed 40-70% positive staining for α2,3SA-gal receptors. In contrast, a greater amount of staining for α2,3SA-gal (50-80%) and α2,6SA-gal (20-50%) receptors were observed along the epithelium of small and large intestine of chickens. Kidney and esophagus sections from the 3 bird species also expressed both avian and human type receptors. In other tissues examined, brain, breast muscles, bursa, spleen, cecal tonsils and oviduct, human type receptors were absent. Though different viral and receptor components may play roles in successful viral replication and transmission, understanding the receptor types and distribution in different tissues of domestic birds might be good initial tool to understand host factors that promote successful influenza viral infection.