The bladder acellular matrix graft in a rat chemical cystitis model:: Functional and histologic evaluation
The bladder acellular matrix graft in a rat chemical cystitis model:: Functional and histologic evaluation
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DOI：
10.1016/s0022-5347(05)64746-5
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发表时间：
2002-08-01
影响因子：
6.6
通讯作者：
Tanagho, EA
中科院分区：
文献类型：
--
作者：
Çayan, S;Chermansky, C;Tanagho, EA
目的：我们探讨了增强在患病膀胱与膀胱脱细胞基质graft.Materials和方法的可行性：在50名女性Sprague-Dawley大鼠化学膀胱炎引起的膀胱灌注盐酸重复每月维持慢性炎症。在诱导化学性膀胱炎后1周对所有大鼠进行尿动力学研究，并在处死时重复。实验组中的29只大鼠接受部分膀胱灌注（50%或更高），随后接受膀胱无细胞基质移植物增强，而21只对照组仅接受每月一次的盐酸灌注。分别于术后2周、1、2、3个月处死大鼠。膀胱被删除，并检查组织学changes.Results：尿动力学研究表明，膀胱容量和顺应性显着高于移植比对照组（p = 0.008和0.006，分别在3个月）。组织学研究显示，1个月时膀胱无细胞基质移植物内的尿路上皮和平滑肌再生，3个月时神经再生。所有移植大鼠移植区肥大细胞数量均明显低于宿主膀胱（p
Purpose: We investigated the feasibility of augmentation in a diseased bladder with a bladder acellular matrix graft.Materials and Methods: In 50 female Sprague-Dawley rats chemical cystitis was induced by intravesical instillation of HCl repeated monthly to maintain chronic inflammation. Urodynamic studies were performed in all rats 1 week after the induction of chemical cystitis and repeated at sacrifice. The 29 rats in the experimental group underwent partial cystectomy (50% or greater), followed by bladder acellular matrix graft augmentation, while the 21 controls underwent monthly HCl instillation only. The rats were sacrificed at 2 weeks, 1, 2 and 3 months, respectively. The bladder was removed and examined for histological changes.Results: Urodynamic studies showed that bladder capacity and compliance were significantly higher in the grafted than in the control group (p = 0.008 and 0.006, respectively, at 3 months). Histological studies revealed urothelial and smooth muscle regeneration within the bladder acellular matrix graft at 1 month and nerve regeneration at 3. The number of mast cells was significantly lower in the grafted region than in the host bladder of all grafted rats (p