A gene signature of nonhealing venous ulcers: potential diagnostic markers.
A gene signature of nonhealing venous ulcers: potential diagnostic markers.
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DOI：
10.1016/j.jaad.2008.07.018
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发表时间：
2008-11
影响因子：
13.8
通讯作者：
Kirsner RS
中科院分区：
文献类型：
--
作者：
Charles CA;Tomic-Canic M;Vincek V;Nassiri M;Stojadinovic O;Eaglstein WH;Kirsner RS
下肢静脉溃疡占所有下肢溃疡的一半以上。人们的极大兴趣集中在了解患者无法通过标准治疗治愈的生理基础上。本研究使用互补 DNA 微阵列分析来自愈合和未愈合的静脉性腿部溃疡的组织样本，以确定这些二分群体的基因表达谱。溃疡大小和慢性程度，这些因素已被确定为愈合的预后指标，用于将腿部静脉溃疡分为愈合与不愈合。从所有腿部静脉性溃疡的伤口边缘和伤口床获取穿刺活检样本。报告了两个伤口群体之间差异表达大于两倍的前 15 个基因 (p < 0.05)。多种基因的表达存在显着差异，其中编码结构表皮蛋白的基因、与过度增殖和组织损伤相关的基因以及转录因子表现出特殊的差异。小样本量可能会减轻研究结果的潜在临床影响。这里显示的基因表达谱可能对慢性腿部静脉溃疡新疗法的开发有影响，也可能作为伤口愈合的预后指标。
Venous leg ulcers are responsible for more than half of all lower extremity ulcerations. Significant interest has been focused on understanding the physiologic basis upon which patients fail to heal with standard therapy. This study uses complementary DNA microarray analysis of tissue samples from healing and non-healing venous leg ulcers to identify the genetic expression profiles from these dichotomous populations. Ulcer size and chronicity, factors that have been identified as prognostic indicators for healing, were used to distribute venous leg ulcers as healing versus non-healing. Punch biopsy samples were obtained from the wound edge and wound bed of all venous leg ulcers. The top fifteen genes with differential expression greater than twofold between the two populations of wounds (p < 0.05) were reported. Significant differences were demonstrated in the expression of a diverse collection of genes, with particular differences demonstrated by genes coding for structural epidermal proteins, genes associated with hyperproliferation and tissue injury, as well as transcription factors. Small sample size may mitigate potential clinical implications of findings. The genetic expression profiles displayed here may have implications for the development of novel therapies for chronic venous leg ulcers, and may also serve as prognostic indicators for wound healing.