Composition of Gut Microbiota Influences Resistance of Newly Hatched Chickens to Salmonella Enteritidis Infection.
Composition of Gut Microbiota Influences Resistance of Newly Hatched Chickens to Salmonella Enteritidis Infection.
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DOI：
10.3389/fmicb.2016.00957
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发表时间：
2016
影响因子：
5.2
通讯作者：
Rychlik I
中科院分区：
文献类型：
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作者：
Varmuzova K;Kubasova T;Davidova-Gerzova L;Sisak F;Havlickova H;Sebkova A;Faldynova M;Rychlik I
由于家禽是人类非伤寒沙门氏菌的一个非常常见的来源，旨在减少鸡中沙门氏菌流行率的不同干预措施被认为是减少人类沙门氏菌病发病率的有效措施。其中一种干预措施是使用益生菌或竞争性排斥产品。在这项研究中，我们测试了来自不同年龄的供体母鸡的微生物群是否能同样保护鸡免受肠炎沙门氏菌感染。因此，将1、3、16、28和42周龄供体的盲肠提取物口服给雏鸡接种，7天后感染肠炎沙门氏菌。4 d后终止实验。在第二个试验中，新孵鸡组分别在出生第1天接种35周龄母鸡盲肠提取物，第2天接种肠链球菌感染，第1天接种肠链球菌感染，第2天接种盲肠提取物治疗性喂养，或在出生第1天接种盲肠提取物和肠链球菌的混合物。试验于鸡5日龄结束。沙门氏菌培养和鸡的基因表达证实，用3周龄或更大的鸡的微生物群接种新孵化的鸡可以保护它们免受肠炎沙门氏菌的攻击。另一方面，来自1周大供体的微生物群未能保护鸡免受肠炎沙门氏菌的攻击。35周龄母鸡的微生物群在给药24小时后仍对鸡有保护作用。然而，同时或治疗性给药的微生物群不能保护鸡免受肠炎沙门氏菌感染。肠道菌群可以作为预防肠炎沙门氏菌感染的措施，但其组成和早期给药对其疗效至关重要。
Since poultry is a very common source of non-typhoid Salmonella for humans, different interventions aimed at decreasing the prevalence of Salmonella in chickens are understood as an effective measure for decreasing the incidence of human salmonellosis. One such intervention is the use of probiotic or competitive exclusion products. In this study we tested whether microbiota from donor hens of different age will equally protect chickens against Salmonella Enteritidis infection. Newly hatched chickens were therefore orally inoculated with cecal extracts from 1-, 3-, 16-, 28-, and 42-week-old donors and 7 days later, the chickens were infected with S. Enteritidis. The experiment was terminated 4 days later. In the second experiment, groups of newly hatched chickens were inoculated with cecal extracts of 35-week-old hens either on day 1 of life followed by S. Enteritidis infection on day 2 or were infected with S. Enteritidis infection on day 1 followed by therapeutic administration of the cecal extract on day 2 or were inoculated on day 1 of life with a mixture of the cecal extract and S. Enteritidis. This experiment was terminated when the chickens were 5 days old. Both Salmonella culture and chicken gene expression confirmed that inoculation of newly hatched chickens with microbiota from 3-week-old or older chickens protected them against S. Enteritidis challenge. On the other hand, microbiota from 1-week-old donors failed to protect chickens against S. Enteritidis challenge. Microbiota from 35-week-old hens protected chickens even 24 h after administration. However, simultaneous or therapeutic microbiota administration failed to protect chickens against S. Enteritidis infection. Gut microbiota can be used as a preventive measure against S. Enteritidis infection but its composition and early administration is critical for its efficacy.