Isolation and biochemical characterizations of the bacteria (Acidovorax avenae subsp. avenae ) associated with red stripe disease of sugarcane
Isolation and biochemical characterizations of the bacteria (Acidovorax avenae subsp. avenae ) associated with red stripe disease of sugarcane
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与甘蔗红条病相关的细菌（Acidovorax avenae subsp. avenae）的分离和生化特性
DOI：
10.5897/ajb11.953
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发表时间：
2011
影响因子：
--
通讯作者：
F. Hafeez
中科院分区：
文献类型：
--
作者：
S. Zia;M. I. Haque;S. M. Mughal;K. N. Shah;A. Irfan;S. Afghan;A. Shahazad;A. Batool;P. Khanum;K. Hussain;K. Nawaz;M. N. Hassan;F. Hafeez
燕麦食酸菌的研究。与甘蔗红条病相关的麦长管蚜的研究在Pir梅尔Ali Shah干旱农业大学拉瓦尔平迪的植物病理学系与巴基斯坦Jhang的Shakarganj糖研究所（SSRI）合作于2009 - 2010年进行。2009年秋植于上海科学技术研究所阿沙巴研究农场的甘蔗优良无性系上发生了红条病。从患病植物中分离细菌。这些分离物在马铃薯葡萄糖琼脂（PDA）培养基上在29°C下产生直径为1.7至1.9 mm的白色凸面菌落，并且在酵母提取物葡萄糖白垩琼脂（YDC）培养基上在27°C下产生直径为1.8至2.0 mm的黄色菌落。细菌在PDA上为棒状，直径为0.5 ~ 0.6 × 1.4 ~ 1.6 μm，在YDC上为0.6 ~ 0.7 × 1.5 ~ 1.7 μm。细菌培养物在不同温度水平下储存150天。在4°C下储存的细菌培养物在27°C下的YDC上150天后显示出最佳结果，而在29°C下的PDA上120天后显示出阳性结果。细菌为革兰氏阴性，柠檬酸盐利用阳性，氧化酶阴性，过氧化氢酶阳性，尿素酶阴性。经形态学观察和生化特性鉴定，该菌为A.燕麦亚种阿维纳。体外筛选各种抗生素抑制A.燕麦亚种在YDC培养基上，氨苄青霉素和万古霉素的效果最好。对甘蔗红条病进行了人工接种试验。对疾病发展进行长达六周的观察。在27个品种中，有16个抗病，4个中抗，5个中感，2个感病。关键词：甘蔗，酵母提取物葡萄糖白垩琼脂（YDC），马铃薯葡萄糖琼脂（PDA），燕麦噬酸菌亚种。燕麦，生化特性，抗生素。
Studies on Acidovorax avenae subsp. avenae, associated with red stripe disease of sugarcane was conducted in the Department of Plant Pathology, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi during 2009 to 2010, in collaboration with Shakarganj Sugar Research Institute (SSRI), Jhang, Pakistan. Red stripe of sugarcane were recently observed on promising clones of sugarcane planted in autumn 2009 at Ashaba Research Farm of SSRI. Bacteria were isolated from diseased plants. These isolates yielded off white convex colonies on potato dextrose agar (PDA) media at 29°C with 1.7 to 1.9 mm diameter and were yellow on yeast extract dextrose chalk agar (YDC) media at 27°C with 1.8 to 2.0 mm diameter. The bacteria were rod shape measuring 0.5 to 0.6 × 1.4 to 1.6 μm on PDA and 0.6 to 0.7 × 1.5 to 1.7 μm on YDC. Bacterial culture was stored at different temperature levels for 150 days. Reisolation of bacterial culture which was stored at 4°C showed best result on YDC at 27°C after 150 days, whereas it showed positive result after 120 days on PDA at 29°C. Bacteria were gram negative, citrate utilization was positive, oxidase was negative, catalase was positive and urease was negative. Morphological appearance and biochemical characterizations identified the bacteria as A. avenae subsp. Avenae . In vitro screening for the efficacy of various antibiotics to inhibit the growth of A. avenae subsp. avenae on YDC media showed that ampicillin and vancomycin were most effective. Artificial inoculation on sugarcane against red stripe disease was observed. Observations were made upto six weeks for disease development. Out of 27 varieties, 16 were found resistant, four moderately resistant, five moderately susceptible and two susceptible. Key words : Sugarcane, yeast extract dextrose chalk agar (YDC), potato dextrose agar (PDA), Acidovorax avenae subsp. avenae, biochemical characterization, antibiotics.