Fabricating and regulating peroxidase-like activity of eggshell membrane-templated gold nanoclusters for colorimetric detection of staphylococcal enterotoxin B.
Fabricating and regulating peroxidase-like activity of eggshell membrane-templated gold nanoclusters for colorimetric detection of staphylococcal enterotoxin B.
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DOI：
10.1016/j.talanta.2018.10.031
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发表时间：
2019-03
期刊：
影响因子：
6.1
通讯作者：
Fang Tan;Xiaoxue Xie;Aiqing Xu;Keqin Deng;Yun-long Zeng;Xiumei Yang;Haowen Huang
中科院分区：
文献类型：
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作者：
Fang Tan;Xiaoxue Xie;Aiqing Xu;Keqin Deng;Yun-long Zeng;Xiumei Yang;Haowen Huang
本研究可以以简便且低成本的方式获得荧光蛋壳膜模板金纳米团簇（Au-ESM）。 Au-ESM的荧光可能会被巯基化合物显着猝灭，并且Au-ESM的过氧化物酶样活性可以通过与谷胱甘肽的反应过程来调节。此外，模拟酶膜的催化活性可以通过免疫反应来调节。根据目标分子调节的催化活性，建立了使用有色金纳米颗粒比色测定葡萄球菌肠毒素 B (SE-B) 的免疫分析方法。该比色法可以检测浓度范围为 0.4–20ng/mL 的 SE-B，检测限 (LOD) 为 0.12ng/mL。作为实际应用，所提出的比色法进一步用于检测面粉、玉米和大米等食品样品中的SE-B，需要非常少的样品量，并且表现出很高的灵敏度和高精度，这为开发具有生物医学和食品安全应用的即时诊断设备提供了有前途的平台。
Fluorescent eggshell membrane-templated gold nanoclusters (Au-ESM) can be obtained in a facile and low-cost manner in this study. The fluorescence of the Au-ESM may be significantly quenched by mercapto-compounds and peroxidase-like activity of Au-ESM could be regulated by the reaction process with glutathione. Moreover, the catalytic activity of the mimetic enzyme membrane could be modulated by immunoreactions. An immunoassay for colorimetric determination of staphylococcal enterotoxins B (SE-B) using colored gold nanoparticles was established based on the catalytic activity adjusted by the target molecules. This colorimetric assay can detect SE-B at the concentration range of 0.4–20 ng/mL and the limit of detection (LOD) is 0.12 ng/mL. As a practical application, the proposed colorimetric assay was further utilized to detect SE-B in food samples such as flour, corn and rice, requiring very low volume of sample and exhibiting great sensitivity and high accuracy, which provides promising platform for development of point-of-care diagnostic devices with biomedical and food safety applications.