Short report:: Polymorphisms in the chloroquine resistance transporter gene in Plasmodium falciparum isolates from Lombok, Indonesia
Short report:: Polymorphisms in the chloroquine resistance transporter gene in Plasmodium falciparum isolates from Lombok, Indonesia
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DOI：
10.4269/ajtmh.2004.71.40
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发表时间：
2004-07-01
影响因子：
3.3
通讯作者：
Kanbara, H
中科院分区：
文献类型：
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作者：
Huaman, MC;Yoshinaga, K;Kanbara, H
在印度尼西亚西龙目岛地区无并发症疟疾患者的 48 株恶性疟原虫中检测了与氯喹耐药相关的恶性疟原虫多药耐药性 1 (pfmdr1) 和恶性疟原虫氯喹耐药转运蛋白 (pfcrt) 基因的多态性。 pfmdr1 中的点突变 N86Y 存在于 35.4% 的分离株中，pfcrt 中的突变 K76T 在除一个研究样本外的所有样本中均发现。已鉴定的 pfcrt 单倍型主要与巴布亚新几内亚型 SagtVMNT 相同（48 株中的 42 株，87.5%），少数分离株具有东南亚型 CVIET（48 株中的 5 株，10.4%）。此外，一种恶性疟原虫分离株带有 K76N 突变，产生单倍型 CVMNN，这在之前的野外分离株中未曾报道。我们的研究结果表明，该地区的氯喹耐药性可能与巴布亚新几内亚的起源相同。
The polymorphisms in the Plasmodium falciparum multidrug resistance 1 (pfmdr1) and P. falciparum chloroquine resistance transporter (pfcrt) genes, which are associated with chloroquine resistance, were examined in 48 P. falciparum isolates from uncomplicated malaria patients from the West Lombok District in Indonesia. The point mutation N86Y in pfmdr1 was present in 35.4% of the isolates and mutation K76T in pfcrt was found in all but one of the samples studied. Identified pfcrt haplotypes were mainly identical to the Papua New Guinea type SagtVMNT (42 of 48, 87.5%), and a few isolates had the Southeast Asia type CVIET (5 of 48,10.4%). Moreover, one P. falciparum isolate harbored the K76N mutation, giving rise to the haplotype CVMNN, which was not previously reported in field isolates. Our findings suggest that chloroquine resistance in this area might have the same origin as in Papua New Guinea.