Abscisic acid induces differential expression of genes involved in wound-induced suberization in postharvest tomato fruit
Abscisic acid induces differential expression of genes involved in wound-induced suberization in postharvest tomato fruit
复制标题
脱落酸诱导采后番茄果实伤口诱导木栓化相关基因的差异表达
DOI：
10.1016/s2095-3119(18)62142-2
复制
发表时间：
2018-12-01
影响因子：
4.8
通讯作者：
Luo Zi-sheng
中科院分区：
文献类型：
--
作者：
Han Xue-yuan;Mao Lin-chun;Luo Zi-sheng
果实在收获和运输过程中发生的损伤需要快速木栓化作为愈合过程的主要部分，以防止感染和脱水。本研究旨在探讨脱落酸（阿坝）对番茄果实创伤诱导木栓化的调控作用，并分析创伤诱导木栓化过程中相关基因的表达谱。果实失重和硬度的测定结果表明，创伤诱导的木栓化在创伤后2d开始。阿坝促进了木栓化过程，并通过自发荧光显微镜和苏丹IV染色观察到木栓化过程中木栓素多酚（SPP）和多酚类物质（SPA）的积累。与SPP合成有关的SlPAL 5和Sl4CL的表达分别在伤后4 d和8 d达到最高。与SPA生物合成相关的SlLACS 1和SlLACS 2分别在阿坝处理的第8 d和第6 d表达量最高。在阿坝处理后2d，SlKCSs、SlCYP86B1、SlFAR3和SlGPATs的转录水平上调。多酚氧化酶和脂氧合酶的活性也增强伤口诱导的木栓化后阿坝的应用。本研究结果表明，阿坝通过提高番茄果实采后相关基因的转录水平，加速了伤口诱导的木栓化进程。
Fruit wounding occurred at harvest and transportation requires rapid suberization as a major part of the healing process to prevent infection and desiccation. The focus of this work was to explore the mediation of abscisic acid (ABA) on wound-induced suberization and to determine expression profiles of specific genes involved in wound-induced suberization in tomato fruit. The measurements of weight loss and fruit firmness suggested wound-induced suberization started at 2 d after wounding. The suberization process with the accumulation of suberin polyphenolics (SPP) and polyaliphatics (SPA) observed through autofluorescence microscopy and Sudan IV staining was accelerated by ABA. Expressions of SlPAL5 and Sl4CL involved in the synthesis of SPP reached the highest at 4 and 8 d after wounding following ABA application, respectively. Associated with SPA biosynthesis, SlLACS1 and SlLACS2 showed the most abundant transcripts at 8 and 6 d in ABA group, respectively. Transcript levels including SlKCSs, SlCYP86B1, SlFAR3, and SlGPATs were up-regulated at 2 d after wounding by ABA. Activities of polyphenol oxidase and lipoxygenase were also enhanced during wound-induced suberization following ABA application. The results in this study proved that ABA accelerated the wound-induced suberization progress by increasing the transcript levels of relevant genes in postharvest tomato fruit.