Effect of human recombinant erythropoietin on human marrow megakaryocyte colony formation in vitro
Effect of human recombinant erythropoietin on human marrow megakaryocyte colony formation in vitro
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人重组促红细胞生成素对体外人骨髓巨核细胞集落形成的影响
DOI：
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发表时间：
1987
影响因子：
6.5
通讯作者：
Otho L. Armstrong
中科院分区：
文献类型：
--
作者：
E. Dessypris;J. H. Gleaton;Otho L. Armstrong
采用血浆凝血法观察重组人促红细胞生成素(EP)对人骨髓巨核细胞集落形成单位(CFU-M)体外生长的影响。EP作为单个刺激因子或作为最佳白细胞条件培养液浓度(PHA-LCM)的附加因子，对CFU-M集落数无影响。在含有亚适浓度PHA-LCM的培养体系中加入EP(0.5-1U/ml)，巨核细胞集落形成率增加50-90%，但对粒-单核细胞集落数(CFU-GM)无明显影响。骨髓细胞在液体悬浮培养中暴露于EP 24-48小时，然后去除激素并检测血浆凝块中CFU-M的细胞数，可使体外巨核细胞集落形成增加50-100%。培养第3天后加入EP后，EP对CFU-M体外生长的促进作用消失。人血清和PHA-LCM中存在的因子是激素在体外对人CFU-M生长发挥增强作用的绝对要求。重组EP能促进人骨髓CFU-M的生长，这种作用似乎发生在CFU-M体外发育的早期阶段。
The effect of human recombinant erythropoietin (EP) on the growth of human marrow megakaryocyte colony forming units (CFU‐M) in vitro was investigated by the use of a plasma clot assay. EP as a single stimulating factor or as an additional factor to optimal concentration of leucocyte conditioned medium (PHA‐LCM) had no effect on the number of CFU‐M derived colonies. However, addition of EP (O·5‐1 U/ml) to cultures with suboptimal concentrations of PHA‐LCM increased megakaryocyte colony formation by 50‐90% but had no effect on the number of granulocytic‐monocytic colonies (CFU‐GM). Exposure of marrow cells to EP for 24‐48 h in liquid suspension cultures, followed by removal of the hormone and assaying the cells for CFU‐M in plasma clots, resulted in a 50‐100% increase of megakaryocyte colony formation in vitro. The augmenting effect of EP on CFU‐M growth in vitro was abolished when EP was added to the medium after the third day of culture. The presence of factors in human serum and in PHA‐LCM was an absolute requirement for the hormone to exert its potentiating effect on human CFU‐M growth in vitro. Recombinant EP potentiates the growth of human marrow CFU‐M and this effect seems to be exerted during the early stages of CFU‐M development in vitro.
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DOI：
10.1056/nejm198309223091202
发表时间：
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期刊：
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影响因子：
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作者：
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影响因子：
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作者：
Mazur,EM;Hoffman,R;Chasis,J;Marchesi,S;Bruno,E
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DOI：
10.1172/jci109983
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影响因子：
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作者：
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