Characterization of Haemaphysalis longicornis recombinant cement-like antigens and preliminary study of their vaccination effects
Characterization of Haemaphysalis longicornis recombinant cement-like antigens and preliminary study of their vaccination effects
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DOI：
10.1292/jvms.68.1289
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发表时间：
2006-12-01
影响因子：
1.2
通讯作者：
Fujisaki, Kozo
中科院分区：
文献类型：
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作者：
Harnnoi, Thasaneeya;Watchabunsook, Songwut;Fujisaki, Kozo
从长角血蜱唾液腺cDNA文库中获得了两个编码免疫优势抗原的基因hlim2和hlim3。重组蛋白在大肠杆菌中以GST融合蛋白的形式表达，并用于免疫。我们观察到，在摄食GST-hlim3免疫的小鼠的最初几天，投喂GST-hlim3免疫的小鼠的若虫附着率显著低于对照组。然而，GST-hlim3免疫并不影响硬蜱的充血率。与之形成鲜明对比的是，GST-hlim2不影响硬蜱的附着率和取食期，但显著减少了吞噬的体重。这些数据突显了两种重组水泥样蛋白在鸡尾酒疫苗中的适用性。
Two genes encoding immunodominant antigens, hlim2 and hlim3, were obtained from a salivary gland cDNA library of the hard tick, Haemaphysalis longicornis. The recombinant proteins were expressed in Escherichia coli as the GST fusion protein and used for immunization. We observed that the attachment rate of nymphal ticks fed on mice immunized with GST-hlim3 was significantly lower than that in the control group during the initial days of feeding. However, immunization with GST-hlim3 did not affect the engorgement rate of the ticks. In sharp contrast, GST-hlim2 did not influence the attachment rate and feeding period of ticks but had a significant reduction in the engorgement body weight. These data highlight the suitability of the 2 recombinant cement-like proteins for use in a cocktail vaccine.