Intergenic transcription and transinduction of the human beta-globin locus
Intergenic transcription and transinduction of the human beta-globin locus
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DOI：
10.1101/gad.11.19.2494
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发表时间：
1997-10-01
影响因子：
10.5
通讯作者：
Proudfoot, NJ
中科院分区：
文献类型：
--
作者：
Ashe, HL;Monks, J;Proudfoot, NJ
我们已经确定了新的核转录本在人类β-珠蛋白基因座使用核运行分析红细胞系和原位杂交分析红细胞组织。这些转录本延伸穿过LCR和基因间区域，但在非红系细胞中检测不到。令人惊讶的是，β-珠蛋白基因（β-珠蛋白、γ-珠蛋白或β-珠蛋白）的瞬时转染诱导LCR和基因间区域从非红细胞系中的染色体β-珠蛋白基因座转录。然而，β-珠蛋白基因本身在转录上保持沉默。诱导依赖于转染质粒中珠蛋白基因的转录，但不需要蛋白质表达。使用原位杂交分析，我们表明，质粒共定位与内源性β-珠蛋白位点提供深入了解转诱导的机制。
We have identified novel nuclear transcripts in the human beta-globin locus using nuclear run-on analysis in erythroid cell lines and in situ hybridization analysis of erythroid tissue. These transcripts extend across the LCR and intergenic regions but are undetectable in nonerythroid cells. Surprisingly, transient transfection of a beta-globin gene (epsilon, gamma, or beta) induces transcription of the LCR and intergenic regions from the chromosomal beta-globin locus in nonerythroid cell lines. The beta-globin genes themselves, however, remain transcriptionally silent. Induction is dependent on transcription of the globin gene in the transfected plasmid but does not require protein expression. Using in situ hybridization analysis, we show that the plasmid colocalizes with the endogenous beta-globin locus providing insight into the mechanism of transinduction.