Effect of mouse strain and diet on feasibility of MRI-based cell tracking in the liver.
Effect of mouse strain and diet on feasibility of MRI-based cell tracking in the liver.
复制标题
小鼠品系和饮食对基于 MRI 的肝脏细胞追踪可行性的影响。
DOI：
10.1002/mrm.28081
复制
发表时间：
2020
影响因子：
3.3
通讯作者：
Shapiro,ErikM
中科院分区：
文献类型：
--
作者：
Mallett,ChristianeL;Hix,JeremyML;Kiupel,Matti;Shapiro,ErikM
目的基于MRI的细胞跟踪识别具有低强度体素的磁性标记细胞的位置。在这里，我们证明了一个应变依赖性的影响，肝脏MRI背景的可行性MRI为基础的细胞跟踪移植细胞在小鼠liver.MethodsFVB小鼠（GFP-LUC和NOG）和C57 BL/6小鼠（GFP+和野生型）喂养3种不同的饮食与不同的铁含量。在不同年龄采集肝脏的体内加权图像和地图。将磁标记的癌细胞脾内注射用于肝迁移;然后，通过体内MRI和生物发光成像对小鼠进行成像。在FVBNOG和FVBGFP-LUC小鼠中，这些小鼠的肝脏也通过磁性粒子成像进行离体成像。FVBNOG小鼠随着年龄的增长，肝脏出现斑点状外观，而FVBGFP-LUC小鼠中未出现。 C57 BL/6 GFP小鼠的肝细胞凋亡率不随年龄变化，肝脏保持明亮和均匀。标记的细胞在一些肝脏中无法通过MRI检测到，尽管成功的植入所示的生物发光成像和磁性粒子imaging.Conclusionstrain，饮食和年龄是重要的考虑因素MRI为基础的细胞跟踪在肝脏中。如果必须使用具有过量肝脏铁的模型，则可以考虑替代成像方法，例如磁粒子成像。
PurposeMRI‐based cell tracking identifies the location of magnetically labeled cells with hypointense voxels. Here we demonstrate a strain‐dependent effect of liver MRI background on the feasibility of MRI‐based cell tracking of transplanted cells in the mouse liver.MethodsFVB mice (GFP‐LUC and NOG) and C57BL/6 mice (GFP+ and wild‐type) were fed 3 different diets with varying iron content. In vivo ‐weighted images and maps of the liver were acquired at different ages. Magnetically labeled cancer cells were injected intrasplenically for hepatic migration; then, mice were imaged by in vivo MRI and bioluminescence imaging. Livers were also imaged ex vivo by magnetic particle imaging.Resultsincreased with age in FVBNOGand FVBGFP‐LUCmice that were fed diets sufficient in iron. FVBNOGmice developed a mottled appearance in their livers with age that did not occur in FVBGFP‐LUCmice. was unchanging with age in C57BL/6GFPmice, and the liver remained bright and homogenous. Labeled cells were not detectable by MRI in some livers despite successful engraftment as shown by bioluminescence imaging and magnetic particle imaging.ConclusionStrain, diet, and age are important considerations for MRI‐based cell tracking in the liver. If a model with excessive liver iron must be used, alternative imaging methods such as magnetic particle imaging can be considered.