Kinetics of the tingible body macrophage response in mouse germinal center development and its depression with age.
Kinetics of the tingible body macrophage response in mouse germinal center development and its depression with age.
复制标题
小鼠生发中心发育中可着色体巨噬细胞反应的动力学及其随年龄的抑制。
DOI：
10.1002/ar.1092290412
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Szakal,AK
中科院分区：
文献类型：
--
作者：
Smith,JP;Lister,AM;Tew,JG;Szakal,AK
虽然易染体巨噬细胞（tingible body macrophages，TBM）在生殖中心的发现已有100多年的历史，但其在生殖中心反应中的作用尚不清楚。在这项研究中，定量评估了TBM反应的动力学，并与年轻小鼠中生发中心发育的动力学相关。老年小鼠中的TBM反应（其具有与年龄相关的老年中枢发育抑制; Szakal等人，1990年，进行了比较分析。用人血清白蛋白攻击幼龄和老龄免疫小鼠，并在0、1、3、5、7、10和14天后评价腘淋巴结和腋窝淋巴结。使用辣根过氧化物酶结合花生凝集素，在交替的连续切片中进行组织化学定位。通过染色小体的存在或通过使用单克隆抗体Mac-2的组织化学方法确定相邻切片上每个生发中心的TBM数量。年轻小鼠的淋巴结分析表明，随着先前存在的生发中心的分离，TBM数量减少。TBM在攻击后5天再次出现，并且TBM动力学证实了新生生发中心大小的增加。事实上，从第5天到第10天，每350-450个B细胞维持一个TBM的恒定比例。在陈旧性淋巴结中，在整个生发中心的发育过程中通常不存在TBM。在生发中心发育之前缺乏TBM以及在老年小鼠中缺乏TBM与诱导生发中心反应需要TBM的概念不一致。在淋巴滤泡中响应于抗原刺激而形成生发中心，并代表抗原特异性记忆B细胞的扩增（Coico等，1983年）。抗原在滤泡树突细胞（FDC）的树突表面上的定位和保留似乎在生发中心发育的起始中是重要的（Szakal等人，1988年）。可染体巨噬细胞（tingible body macrophages，TBM）在生发中心发育中的作用尚不清楚。根据定义，TBM在其细胞质中含有许多吞噬的淋巴细胞，是淋巴组织中生发中心的特征（Flemming，1885; Congdon和Goodman，1961; Swartzendruber和Congdon，1963）。最近的免疫组织化学表型分析显示，虽然TBM表达典型的巨噬细胞相关抗原，但它们代表了巨噬细胞的独特亚群，因为它们表达Thy-1.2抗原（Smith等人，1988年）。最初，认为TBM在消除衰竭淋巴细胞中起作用（Congdon和Goodman，1961）。从那时起，已经提出TBM可能是引发生发中心反应所必需的（Kamperdijk等人，1978年，1982年）。尽管我们已经表明TBM表达Ia抗原（Smith等人，1988），其是抗原呈递所必需的（齐格勒和Unanue，1981;艾伦和
Although tingible body macrophages (TBM) have been recognized in germinal centers for over 100 years, their role in the germinal center response is not clear. In this study, the kinetics of the TBM response was quantitatively assessed and correlated with the kinetics of germinal center development in young mice. The TBM response in old mice (which have an age-related depression of germinal center development; Szakal et al., 1990) was analyzed for comparison. Young and old immune mice were challenged with human serum albumin and 0, 1, 3, 5, 7, 10, and 14 days later the popliteal and axillary lymph nodes were evaluated. Germinal centers were localized histochemically in alternate serial sections using horseradish peroxidase conjugated peanut agglutinin. TBM numbers were determined per germinal center on adjacent sections by the presence of tingible bodies or histochemically by using the monoclonal antibody Mac-2. Analysis of lymph nodes from young mice showed that TBM numbers decreased with the dissociation of preexisting germinal centers. TBM reappeared 5 days after challenge and the TBM kinetics paralleled the increase in size of de novo germinal centers. In fact, a constant ratio of one TBM to every 350-450 B cells was maintained from day 5 to day 10. In old lymph nodes, TBM were generally absent throughout germinal center development. The lack of TBM prior to germinal center development and their absence in aged mice are inconsistent with the concept that TBM are required for the induction of the germinal center reaction. However, the data are consistent with a role for TBM in regulating the magnitude of the germinal center reaction.Germinal centers develop in lymphoid follicles in response to antigenic stimulation and represent the expansion of antigen specific menory B cells (Coico et al., 1983). Localization and retention of antigen on the dendritic surface of follicular dendritic cells (FDC) appears to be important in the initiation of germinal center development (Szakal et al., 1988). The role of tingible body macrophages (TBM) in germinal center development is not clear. By definition, TBM contain many phagocytosed lymphocytes in their cytoplasm and are a characteristic feature of germinal centers in lymphoid tissues (Flemming, 1885; Congdon and Goodman, 1961; Swartzendruber and Congdon, 1963). Recent immunohistochemical phenotypic analysis revealed that while TBM express typical macrophage associated antigens, they represent a unique subpopulation of macrophages in that they express the Thy-1.2 antigen (Smith et al., 1988). Originally, TBM were thought to function in the elimination of effete lymphocytes (Congdon and Goodman, 1961). Since then it has been suggested that TBM may be necessary to initiate the germinal center reaction (Kamperdijk et al., 1978, 1982). Although we have shown that TBM express Ia antigens (Smith et al., 1988), which are necessary for antigen presentation (Ziegler and Unanue, 1981; Allen and