Cytocidal activity and proliferative ability of macrophages infiltrating the EMT6 tumor
Cytocidal activity and proliferative ability of macrophages infiltrating the EMT6 tumor
复制标题
浸润EMT6肿瘤的巨噬细胞的杀细胞活性和增殖能力
DOI：
10.1002/ijc.2910220208
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发表时间：
1978
影响因子：
6.4
通讯作者：
K. L. Beetham
中科院分区：
文献类型：
--
作者：
Carleton C. Stewart;K. L. Beetham
选择EMT6小鼠肿瘤用于研究浸润性巨噬细胞的杀细胞活性和增殖能力，因为它在外植培养时具有很高的镀效率。与肿瘤大小无关，直接从肿瘤培养中镀上细胞的镀效率为28±9.3%。在来自肿瘤的贴壁细胞中，巨噬细胞的比例从第7天的27%增加到第28天的47%。用延时电影显微摄影法直接观察来自肿瘤的贴壁细胞，发现巨噬细胞具有细胞杀伤作用。当在L细胞条件培养基中培养时，未发现巨噬细胞集落，尽管大多数肿瘤细胞集落含有巨噬细胞。当小鼠在建立培养之前首先用0.8 mg BCNU处理时，为了减少集落形成肿瘤细胞的频率，大约一半的集落只含有巨噬细胞。这些结果表明，巨噬细胞在该肿瘤中具有细胞杀伤能力和增殖能力。
The EMT6 mouse tumor was selected for use in the study of cytocidal activity and proliferative ability of infiltrating macrophages because of its high plating efficiency when explanted to culture. The plating efficiency for cells directly plated in culture from the tumor was 28±9.3%, irrespective of the size of the tumor. Of the adherent cells derived from the tumor, the fraction that was macrophages increased from 27% at 7 days to 47% at 28 days after initial injection. Time‐lapse cinemicrography was used to directly observe adherent cells derived from the tumor, and macrophages were found to be cytocidal. When grown in the presence of L‐cell conditioned medium no macrophage colonies were found when cultures were established from untreated mice even though most tumor cell colonies contained macrophages. When mice were first treated with 0.8 mg BCNU prior to establishment of the cultures, in order to reduce the frequency of colony‐forming tumor cells, approximately half the colonies found contained only macrophages. These results show that macrophages in this tumor are cytocidal and capable of proliferation.