Cre recombinase-mediated gene deletion in layer 4 of murine sensory cortical areas
Cre recombinase-mediated gene deletion in layer 4 of murine sensory cortical areas
复制标题
DOI：
10.1002/dvg.20393
复制
发表时间：
2008-06-01
期刊：
影响因子：
1.5
通讯作者：
Xu, Baoji
中科院分区：
文献类型：
--
作者：
Liao, Guey-Ying;Xu, Baoji
丘脑皮层输入到第4层携带主要的上升感觉信息到哺乳动物的感觉皮层，是至关重要的功能和可塑性的感觉皮层区。在这里，我们报告的Six 3-cre转基因，选择性地表达在第4层的感觉皮质区，但不是在丘脑中的识别。在成熟的躯体感觉皮层中，由转基因表达的Cre重组酶能够介导绝大多数第4层神经元（包括GABA能中间神经元）中的基因缺失。第4层的基因缺失主要发生在出生后第1周。因此，这种cre转基因为研究第4层神经元中表达的蛋白质的作用提供了有用的工具。
Thalamocortical input to layer 4 carries the major ascending sensory information to the mammalian sensory cortex and is crucial for the function and plasticity of sensory cortical areas. Here we report identification of a Six3-cre transgene that is selectively expressed in layer 4 of sensory cortical areas but not in the thalamus. In the mature somatosensory cortex Cre recombinase expressed from the transgene is able to mediate gene deletion in the overwhelming majority of layer 4 neurons, including GABAergic interneurons. The gene deletion in layer 4 mainly occurs during the first postnatal week. This cre transgene therefore provides a useful tool for examining the role of proteins expressed in layer 4 neurons.