A new vascular polyester prosthesis impregnated with cross-linked dextran
A new vascular polyester prosthesis impregnated with cross-linked dextran
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DOI：
10.1163/156856202320401997
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发表时间：
2002-01-01
影响因子：
3.6
通讯作者：
Jozefonvicz, J
中科院分区：
文献类型：
--
作者：
Machy, D;Carteron, P;Jozefonvicz, J
为了防止血液通过移植物壁渗漏，必须在植入前对合成血管移植物进行预凝。我们已经用交联葡聚糖浸渍了针织聚酯假体。本研究的目的是开发一种获得不渗透假体的方法，并将这种葡聚糖浸渍移植物的特性与市售胶原浸渍移植物的特性进行比较。这种新的人造血管用葡聚糖涂层;三偏磷酸钠用作交联剂。为了确定浸渍的最佳条件，在交联反应期间测量葡聚糖溶液的动态粘度。结果表明，动态粘度与葡聚糖、氢氧化钠和三偏磷酸钠的浓度相关。研究了温度对动态粘度的影响。将葡聚糖浸渍的移植物的透水性、涂层重量和结构与胶原浸渍的假体进行比较。通过葡聚糖浸渍降低血管移植物的透水性，从对照的1010 ml/min/cm（2）降低至标准测试条件下的0.04 ml/min/cm（2）。葡聚糖涂层能够使移植物不透水。用葡聚糖处理的移植物的涂层重量与胶原浸渍的移植物的重量大致相同。最后，使用扫描电子显微镜分析假体壁的形态。促进内皮细胞的恢复，只观察到与葡聚糖或胶原处理的聚酯移植。
It is essential that a synthetic vascular graft is preclotting prior to implantation in order to prevent blood leaking through the graft wall. We have impregnated a knitted polyester prosthesis with cross-linked dextran. The aim of this study was to develop a process for obtaining an impervious prosthesis and to compare the characteristics of this dextran-impregnated graft with those of a commercially available collagen-impregnated graft. This new vascular prosthesis was coated with dextran; sodium trimetaphosphate was utilized as the cross-linking agent. In an attempt to determine the optimal conditions for impregnation, the dynamic viscosity of the dextran solution was measured during the cross-linking reaction. The results suggest that the dynamic viscosity is correlated with the concentrations of dextran, sodium hydroxide, and sodium trimetaphosphate. The effect of temperature on the dynamic viscosity was also investigated. The water permeability, the coating weight, and the structure of the dextran-impregnated graft were compared with those of a collagen-impregnated prosthesis. The water permeability of the vascular grafts was reduced by dextran impregnation, from 1010 ml/min per cm(2) for the control to 0.04 ml/min per cm(2) under standard testing conditions. The dextran coating is capable of rendering the graft impervious to water. The coating weight of the graft treated with dextran was approximately the same as the weight of the collagen-impregnated graft. Finally, the morphology of the prosthetic wall was analyzed using scanning electron microscopy. The promotion of endothelial cell recovery was only observed for the polyester grafts treated with dextran or collagen.