AGEPC (platelet activating factor) induced stimulation of rabbit platelets: effects on phosphatidylinositol, di- and triphosphoinositides and phosphatidic acid metabolism.
AGEPC (platelet activating factor) induced stimulation of rabbit platelets: effects on phosphatidylinositol, di- and triphosphoinositides and phosphatidic acid metabolism.
复制标题
AGEPC（血小板激活因子）诱导兔血小板刺激：对磷脂酰肌醇、二磷酸肌醇和三磷酸肌醇以及磷脂酸代谢的影响。
DOI：
10.1016/0006-291x(82)91767-3
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发表时间：
1982
影响因子：
3.1
通讯作者：
Hanahan,DJ
中科院分区：
文献类型：
--
作者：
Shukla,SD;Hanahan,DJ
用AGEPC（1-O-烷基-2-乙酰基-sn-甘油基-3-磷酸胆碱）刺激洗涤过的兔血小板，在15秒内导致其磷脂酰肌醇水平降低15-20%，而不影响其他主要类别的磷脂。在相同的时间范围内，磷脂酸（PA）的水平急剧增加约四倍。LysoGEPC在刺激兔血小板方面无活性，不会引起PI或PA的任何变化。在AGEPC刺激血小板过程中加入[32 Pi]后，PI-4-磷酸（DPI）、PI-4，5-二磷酸（TPI）和PA的掺入量在1分钟内显著增加，而PI的掺入量在1分钟后才增加。这些结果清楚地表明，AGEPC诱导的兔血小板的刺激与肌醇磷脂和磷脂酸的代谢有关。这些发现的相关性AGEPC和Ca 2+动员的作用方式进行了讨论。
Stimulation of washed rabbit platelets with AGEPC (1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine) caused a 15–20% decrease in their phosphatidylinositol level within 15 seconds without affecting other major classes of phospholipids. In the same time frame the level of phosphatidic acid (PA) increased dramatically some four fold. LysoGEPC, which is inactive in stimulating rabbit platelets, did not cause any change in PI or PA. When [32 Pi] was present during the stimulation of platelets by AGEPC, the incorporation of radiolabel into PI-4-phosphate (DPI), PI-4, 5-bis phosphate (TPI) and PA was enhanced significantly within one minute while the incorporation into PI increased only after one minute. These results clearly established that AGEPC induced stimulation of rabbit platelets was associated with the metabolism of inositol phospholipids and phosphatidic acid. The relevance of these findings to the mode of action of AGEPC and Ca 2+ mobilization is also discussed.