Complete genome sequence of Salmonella enterica serovar typhimurium LT2
Complete genome sequence of Salmonella enterica serovar typhimurium LT2
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DOI：
10.1038/35101614
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发表时间：
2001-10-25
期刊：
影响因子：
64.8
通讯作者：
Wilson, RK
中科院分区：
文献类型：
--
作者：
McClelland, M;Sanderson, KE;Wilson, RK
肠道沙门氏菌亚种I、鼠伤寒血清型（S.鼠伤寒沙门氏菌），是人类胃肠炎的主要原因，并被用作人类伤寒的小鼠模型（1）。非伤寒沙门氏菌病的发病率在世界范围内不断增加（2-4），每年在人群中造成数百万感染和许多死亡。本研究测定了4，857-kb的S.鼠伤寒沙门氏菌LT 2。S.使用先前完成的三种相关细菌的基因组，测定了八种相关肠杆菌中的鼠伤寒沙门氏菌LT 2基因，甲型副伤寒沙门氏菌（S.甲型副伤寒）和肺炎克雷伯氏菌，以及三个未测序的基因组与S.鼠伤寒LT 2基因。基因的横向转移是频繁的，11%的S。鼠伤寒沙门氏菌LT 2基因缺失。伤寒沙门氏菌（S.伤寒），大肠杆菌K12缺失29%。352个基因同源物的S。typhimurium LT 2局限于S.肠道病原体--含有大多数哺乳动物和鸟类病原体（5）--可用于流行病学、宿主特异性和发病机理的研究。这些同源物中的大多数以前是未知的，并且50个可能被输出到周质或外膜，使它们可作为治疗或疫苗靶点。
Salmonella enterica subspecies I, serovar Typhimurium (S. typhimurium), is a leading cause of human gastroenteritis, and is used as a mouse model of human typhoid fever(1). The incidence of non-typhoid salmonellosis is increasing worldwide(2-4), causing millions of infections and many deaths in the human population each year. Here we sequenced the 4,857-kilobase (kb) chromosome and 94-kb virulence plasmid of S. typhimurium strain LT2. The distribution of close homologues of S. typhimurium LT2 genes in eight related enterobacteria was determined using previously completed genomes of three related bacteria, sample sequencing of both S. enterica serovar Paratyphi A (S. paratyphi A) and Klebsiella pneumoniae, and hybridization of three unsequenced genomes to a microarray of S. typhimurium LT2 genes. Lateral transfer of genes is frequent, with 11% of the S. typhimurium LT2 genes missing from S. enterica serovar Typhi (S. typhi), and 29% missing from Escherichia coli K12. The 352 gene homologues of S. typhimurium LT2 confined to subspecies I of S. enterica- containing most mammalian and bird pathogens(5)- are useful for studies of epidemiology, host specificity and pathogenesis. Most of these homologues were previously unknown, and 50 may be exported to the periplasm or outer membrane, rendering them accessible as therapeutic or vaccine targets.