Red fluorescent proteins: chromophore formation and cellular applications.

Red fluorescent proteins: chromophore formation and cellular applications.
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DOI:
10.1016/j.sbi.2012.09.002
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发表时间:
2012-10
影响因子:
6.8
通讯作者:
Verkhusha VV
Verkhusha VV
中科院分区:
生物学2区
文献类型:
--
作者:
Miyawaki A;Shcherbakova DM;Verkhusha VV

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在过去的十年中,许多发出橙色、红色和远红色荧光的红色荧光蛋白(RFP)已从珊瑚动物中分离出来,并通过定向分子进化得到发展。一些 RFP 具有的一个吸引人的特性是它们的红色荧光可以通过特定波长的照明来打开或调制。 RFP 开发的最新进展伴随着发色团化学的详细研究。彻底了解红色发色团翻译后修饰涉及的分子机制将使科学家能够设计具有所需特性的 RFP,以推进成像应用。本文提供了有关 RFP 的化学和应用的广阔视角。
In the last decade, a number of red fluorescent proteins (RFPs) that emit orange, red, and far-red fluorescence have been isolated from anthozoans (corals), and developed through directed molecular evolution. An attractive property possessed by some RFPs is that their red fluorescence can be turned on or modulated by illumination at specific wavelengths. Recent progress in the development of RFPs has been accompanied with detailed studies of chromophore chemistry. A thorough understanding of the molecular mechanisms involved in the post-translational modifications of red chromophores would enable scientists to design RFPs with the desired properties to advance imaging applications. This article provides a broad perspective on the chemistry and applications of RFPs.
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