Fluorescence resonance energy transfer
Fluorescence resonance energy transfer
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DOI：
10.1016/0958-1669(95)80016-6
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发表时间：
1995-01-01
影响因子：
7.7
通讯作者：
Clegg, Robert M.
中科院分区：
文献类型：
--
作者：
Clegg, Robert M.
在过去的一年中，许多研究证明了荧光共振能量转移作为一种技术的实用性，可用于探测复杂的分子间相互作用，并确定由不同分子成分（如蛋白质、脂质、碳水化合物、核酸，甚至病毒细胞）组成的多组分结构的空间延伸和几何特征。对于需要高灵敏度、特异性、非侵入性、快速和相对简单的测量的研究人员来说，荧光共振能量转移的好处变得越来越明显。
In the past year, a number of studies have demonstrated the utility of fluorescence resonance energy transfer as a technique for probing complex intermolecular interactions and for determining the spatial extension and geometrical characteristics of multicomponent structures composed of diverse molecular constituents, such as proteins, lipids, carbohydrates, nucleic acids, and even cells with viruses. The benefits of fluorescence resonance energy transfer are becoming increasingly evident to researchers who require measurements with high sensitivity, specificity, non-invasiveness, rapidity, and relative simplicity.