The COP9 signalosome is an essential regulator of development in the filamentous fungus Aspergillus nidulans
The COP9 signalosome is an essential regulator of development in the filamentous fungus Aspergillus nidulans
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DOI：
10.1046/j.1365-2958.2003.03612.x
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发表时间：
2003-08-01
影响因子：
3.6
通讯作者：
Braus, GH
中科院分区：
文献类型：
--
作者：
Busch, S;Eckert, SE;Braus, GH
COP9信号体（CSN）是一种保守的多蛋白复合物，参与真核生物发育的调控。构巢曲霉两个基因csnD和csnE推导的氨基酸序列与高等真核生物的第四和第五CSN亚基具有高度一致性。csnD转录本在营养生长和发育过程中是丰富的，并且相应的蛋白质在细胞核中积累。缺失任何一个csn基因的菌株都是活的，并且在允许发育的条件下显示出相同的突变表型：菌丝部分呈现红色，并且含有尺寸减小的细胞。此外，传播开始的光依赖性受到影响。Deltacsn突变体能够启动性周期并发育原基，但成熟为有性子实体被阻断。这种发育停滞不能通过过度表达性激活物天鹅绒（VEA）来克服。我们的结论是，COP9信号体在A. nidulans是性发育的关键调节因子，并且其提出的对高等真核生物的CSN的结构和功能保守性使得能够在遗传上顺从的生物体中研究这种调节复合物。
The COP9 signalosome (CSN) is a conserved multiprotein complex involved in regulation of eukaryotic development. The deduced amino acid sequences of two Aspergillus nidulans genes, csnD and csnE, show high identities to the fourth and fifth CSN subunits of higher eukaryotes. The csnD transcript is abundant during vegetative growth as well as development and the corresponding protein accumulates in the nucleus. Strains deleted for either csn gene are viable and show identical mutant phenotypes at conditions that allow development: hyphae appear partly red and contain cells of reduced size. Additionally, light dependence of propagation onset is affected. The Deltacsn mutants are capable of initiating the sexual cycle and develop primordia, but maturation to sexual fruit bodies is blocked. This developmental arrest could not be overcome by overexpression of the sexual activator velvet (VEA). We conclude that the COP9 signalosome in A. nidulans is a key regulator of sexual development, and its proposed structural and functional conservation to the CSN of higher eukaryotes enables studies on this regulatory complex in a genetically amenable organism.