CaDHN5, a Dehydrin Gene from Pepper, Plays an Important Role in Salt and Osmotic Stress Responses
CaDHN5, a Dehydrin Gene from Pepper, Plays an Important Role in Salt and Osmotic Stress Responses
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DOI：
10.3390/ijms20081989
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Rugang Chen
中科院分区：
文献类型：
--
作者：
Dan Luo;Xiaoming Hou;Yumeng Zhang;Yuancheng Meng;Huafeng Zhang;Suya Liu;Xinke Wang;Rugang Chen
Dehydrins (DHNs), as a sub-family of group two late embryogenesis-abundant (LEA).proteins, have attracted considerable interest owing to their functions in enhancing abiotic stress.tolerance in plants. Our previous study showed that the expression of CaDHN5 (a dehydrin gene from.pepper) is strongly induced by salt and osmotic stresses, but its function was not clear. To understand.the function of CaDHN5 in the abiotic stress responses, we produced pepper (Capsicum annuum L.).plants, in which CaDHN5 expression was down-regulated using VIGS (Virus-induced Gene Silencing),.and transgenic Arabidopsis plants overexpressing CaDHN5. We found that knock-down of CaDHN5.suppressed the expression of manganese superoxide dismutase (MnSOD) and peroxidase (POD).genes. These changes caused more reactive oxygen species accumulation in the VIGS lines than.control pepper plants under stress conditions. CaDHN5-overexpressing plants exhibited enhanced.tolerance to salt and osmotic stresses as compared to the wild type and also showed increased.expression of salt and osmotic stress-related genes. Interestingly, our results showed that many.salt-related genes were upregulated in our transgenic Arabidopsis lines under salt or osmotic stress..Taken together, our results suggest that CaDHN5 functions as a positive regulator in the salt and.osmotic stress signaling pathways.
