ESTIMATION OF FLUX CONTROL COEFFICIENTS FROM INHIBITOR TITRATIONS BY NONLINEAR-REGRESSION
ESTIMATION OF FLUX CONTROL COEFFICIENTS FROM INHIBITOR TITRATIONS BY NONLINEAR-REGRESSION
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DOI：
10.1016/0014-5793(90)81355-r
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发表时间：
1990-11-12
期刊：
影响因子：
3.5
通讯作者：
BOHNENSACK, R
中科院分区：
文献类型：
--
作者：
GELLERICH, FN;KUNZ, WS;BOHNENSACK, R
A mathematical model was developed to estimate flux control coefficients (Co) from titration studies with specific non-competitive inhibitors. In contrast to the normally used graphical determination the model pays regard to the dissociation equilibrium (kD) that exists between inhibitor and its binding sites (Eo) as well as to an objective estimation of the initial slope. The model was used for the analysis of titration experiments where the respiration of rat liver mitochondria was inhibited with carboxyatractyloside and antimycin A. It is shown that the graphical estimation ofEoandColead to significant overestimation if the ratioKd/Eois larger than 10−4which can be avoided by using our model.
