NG2 proteoglycan‐expressing microglia as multipotent neural progenitors in normal and pathologic brains
NG2 proteoglycan‐expressing microglia as multipotent neural progenitors in normal and pathologic brains
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DOI：
10.1002/glia.20332
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发表时间：
2006-05
期刊：
影响因子：
6.2
通讯作者：
A. Yokoyama;Aiko Sakamoto;K. Kameda;Y. Imai;Junya Tanaka
中科院分区：
文献类型：
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作者：
A. Yokoyama;Aiko Sakamoto;K. Kameda;Y. Imai;Junya Tanaka
大鼠原代小胶质细胞（MG）通过在10%和70%血清补充培养基中两步培养5天获得了产生神经外胚层细胞的多能特性。这种多能MG，称为前小胶质母细胞（ProMGBs），形成细胞聚集体，在转移到无血清培养基后不久产生具有神经外胚层表型的细胞。免疫组化结果显示，新生大鼠脑内有少量MG表达NG 2硫酸软骨素蛋白聚糖（NG 2）。来自新生儿脑的原代培养物含有NG 2 + MG，其似乎是NG 2 + ProMGB聚集体的来源。聚集体为MG标志物+/NG 2 +/GFAP+/NCAM+/S-100β-，并具有碱性磷酸酶活性。NG 2 + MG的显著积累被观察到接近成熟大鼠脑中的刺伤。累积的NG 2 + MG在伤口的数量逐渐减少，但细胞持续到150天后损伤。此外，GFAP免疫反应性在伤口周围显著增加。用胰蛋白酶-EDTA分离的伤口中的NG 2 + MG在70%血清补充培养基中形成NG 2+聚集体，然后在无血清培养基中转化为具有神经外胚层表型的细胞。虽然很难从成熟的大脑中分离出有活力的神经元，但来自刺伤的细胞在体外很容易产生携带β-微管蛋白III+细胞的细胞。这些数据表明，NG 2 + MG在正常发育或病理性脑中参与脑的发生或再生。© 2006 Wiley利斯公司
Rat primary microglia (MG) acquired a multipotent property to give rise to neuroectodermal cells through two‐step culture in 10 and 70% serum‐supplemented media for 5 days. Such multipotent MG, called promicroglioblasts (ProMGBs), formed cell aggregates, which generated cells with neuroectodermal phenotypes shortly after their transfer into serum‐free medium. As revealed by immunohistochemistry, there were a few MG expressing NG2 chondroitin sulfate proteoglycan (NG2) in the neonatal rat brain. Primary culture from the neonatal brain contained NG2+ MG, which appeared to be the source of NG2+ ProMGB aggregates. The aggregates were MG marker+/NG2+/GFAP+/NCAM+/S‐100β− and had alkaline phosphatase activity. The marked accumulation of NG2+ MG was observed close to stab wounds made in the mature rat brain. The accumulated NG2+ MG in the wound gradually decreased in number, but the cells persisted up to 150 days postlesioning. In addition, GFAP immunoreactivity increased markedly around the wound. The NG2+ MG in the wounds separated with trypsin‐EDTA formed NG2+ aggregates in 70% serum‐supplemented medium and then transformed into cells with neuroectodermal phenotypes in serum‐free medium. Although it is difficult to separate viable neurons from mature brains, cells from stab wounds generated process‐bearing β‐tubulin III+ cells in vitro easily. These data suggest that NG2+ MG in normal developing or pathologic brains are involved in the genesis or regeneration of the brain. © 2006 Wiley‐Liss, Inc.