Glutamine supplementation improves intestinal cell proliferation and stem cell differentiation in weanling mice.
Glutamine supplementation improves intestinal cell proliferation and stem cell differentiation in weanling mice.
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DOI：
10.29219/fnr.v62.1439
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发表时间：
2018
影响因子：
3.3
通讯作者：
Ren W
中科院分区：
文献类型：
--
作者：
Chen S;Xia Y;Zhu G;Yan J;Tan C;Deng B;Deng J;Yin Y;Ren W
肠干细胞可分化为吸收性肠上皮细胞和分泌性细胞，包括潘氏细胞、杯状细胞和肠内分泌细胞。谷氨酰胺是小肠肠细胞的主要代谢燃料，对于肠细胞的活力和生长至关重要。补充谷氨酰胺是否影响肠道干细胞的分化尚不清楚。三周大的 ICR（癌症研究所）雄性小鼠被随机分为两组：1）接受基础饮食和正常饮用水的小鼠，2）接受基础饮食和补充谷氨酰胺的饮用水的小鼠。 2周后，处死小鼠以收集回肠用于分析。研究发现，断奶小鼠补充谷氨酰胺会降低回肠隐窝深度，导致回肠绒毛与隐窝的比例较高，但会促进肠细胞的细胞增殖和回肠中Lgr5（富含亮氨酸重复序列的g蛋白偶联受体5）的mRNA表达。谷氨酰胺对潘氏细胞和杯状细胞的数量以及吸收性肠上皮细胞、潘氏细胞、杯状细胞和肠内分泌细胞标记物的表达没有影响。这些发现揭示了膳食谷氨酰胺补充剂对改善断奶哺乳动物肠道形态的有益作用。
Intestinal stem cells can be differentiated into absorptive enterocytes and secretory cells, including Paneth cells, goblet cells, and enteroendocrine cells. Glutamine is a primary metabolic fuel of small intestinal enterocytes and is essential for the viability and growth of intestinal cells. Whether glutamine supplementation affects the differentiation of intestinal stem cells is unknown. Three-week-old ICR (Institute of Cancer Research) male mice were divided randomly into two groups: 1) mice receiving a basal diet and normal drinking water and 2) mice receiving a basal diet and drinking water supplemented with glutamine. After 2 weeks, the mice were sacrificed to collect the ileum for analysis. The study found that glutamine supplementation in weanling mice decreases the crypt depth in the ileum, leading to higher ratio of villus to crypt in the ileum, but promotes cell proliferation of intestinal cells and mRNA expression of Lgr5 (leucine-rich repeat-containing g-protein coupled receptor5) in the ileum. Glutamine has no effect on the number of Paneth cells and goblet cells, and the expression of markers for absorptive enterocytes, Paneth cells, goblet cells, and enteroendocrine cells. These findings reveal the beneficial effects of dietary glutamine supplementation to improve intestinal morphology in weanling mammals.