Antioxidative and anticancer activity of extracts of cherry (Prunus serrulata var. spontanea) blossoms
Antioxidative and anticancer activity of extracts of cherry (Prunus serrulata var. spontanea) blossoms
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DOI：
10.1007/s11130-007-0045-9
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发表时间：
2007-06-01
影响因子：
4
通讯作者：
Lee, Seung-Cheol
中科院分区：
文献类型：
--
作者：
Lee, Bo-Bae;Cha, Mi-Ran;Lee, Seung-Cheol
采用甲醇、乙醇、丙酮等有机溶剂提取和水提取方法对樱桃（Prunus serrulata var.密地松）花，并评价提取物的抗氧化活性。100 μ g/ml的Methylethyl CBE具有最高的总酚含量（104.30 μ M）、自由基清除活性（34.2%）和还原力（0.391）。用彗星试验观察CBE对H_2O_2诱导的人白细胞DNA损伤的影响。CBE对200 μ M H_2O_2诱导的DNA损伤有明显的抑制作用，且呈剂量依赖性，其中CBE的甲醇溶液抑制作用最强。500 μ g/ml的甲醇CBE对人结肠癌细胞系HT-29的生长显示出38.8%的抑制。这些结果表明，樱花可以提供有价值的生物活性物质。
Organic solvent (methanol, ethanol, and acetone) extracts and water extracts of cherry (Prunus serrulata var. spontanea) blossoms were prepared, and antioxidant activities of the extracts were evaluated. Methanolic CBE (100 mu g/ml) showed the highest total phenol content (104.30 mu M), radical scavenging activity (34.2%), and reducing power (0.391). The effect of CBE on DNA damage induced by H2O2 in human leukocytes was evaluated by Comet assay. All CBE was a potent dose dependent inhibitor of DNA damage induced by 200 mu M of H2O2, methanolic CBE showed the most strong inhibition activity. The methanolic CBE of 500 mu g/ml showed 38.8% inhibition against growth of human colon cancer cell line HT-29. These results indicated that cherry blossoms could provide valuable bioactive materials.