Structure-based rationale for the rescue of systemic movement of brome mosaic virus by spontaneous second-site mutations in the coat protein gene.
Structure-based rationale for the rescue of systemic movement of brome mosaic virus by spontaneous second-site mutations in the coat protein gene.
复制标题
通过外壳蛋白基因的自发第二位点突变来拯救雀麦花叶病毒的系统运动的基于结构的原理。
DOI：
10.1128/jvi.71.3.2500-2504.1997
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Bujarski,JJ
中科院分区：
文献类型：
--
作者：
Flasinski,S;Dzianott,A;Speir,JA;Johnson,JE;Bujarski,JJ
我们描述了外壳蛋白开放阅读框内的自发第二位点回复，该回复挽救了雀麦花叶病毒突变体的系统传播表型并增加了病毒体稳定性。基于相关豇豆褪绿斑驳病毒的晶体结构，我们发现修饰残基在空间上聚集，影响六聚体和五聚体的形成，从而影响病毒粒子的稳定性。
We describe spontaneous second-site reversions within the coat protein open reading frame that rescue the systemic-spread phenotype and increase virion stability of a mutant of brome mosaic virus. Based on the crystal structure of the related cowpea chlorotic mottle virus, we show that the modified residues are spatially clustered to affect the formation of hexamers and pentamers and therefore virion stability.