Occurrence of 4-hydroxyalkenals in rat tissues determined as pentafluorobenzyl oxime derivatives by gas chromatography-mass spectrometry.
Occurrence of 4-hydroxyalkenals in rat tissues determined as pentafluorobenzyl oxime derivatives by gas chromatography-mass spectrometry.
复制标题
通过气相色谱-质谱法确定大鼠组织中 4-羟基烯醛的存在为五氟苄基肟衍生物。
DOI：
10.1016/s0006-291x(86)80091-2
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
E. Dratz
中科院分区：
文献类型：
--
作者：
F. V. van Kuijk;D. Thomas;R. J. Stephens;E. Dratz
Malondialdehyde measurements have been the major tool for studying relationships between lipid peroxidation and tissue pathology. Recently, we presented a novel gas chromatography-mass spectrometry method for direct detection of phospholipid peroxides with picogram sensitivity based on transesterfication of phospholipids or triglycerides to form pentafluorobenzyl esters. Under some circumstances the reactive primary oxidation products break down. Therefore, we developed a convenient, high sensitivity method to detect more stable secondary lipid oxidation products, the 4-hydroxyalkenals. The method accomplishes a facile extraction of 4-hydroxynonenal from tissues by forming pentafluorobenzyl oxime derivatives to displace aldehydes from Schiff base linkages. 4-hydroxynonenal was found in heart, liver, adrenal, and testis from rats and was detected to the 10–100 pg level by the current method.
