A novel instrument for studying the flow behaviour of erythrocytes through microchannels simulating human blood capillaries
A novel instrument for studying the flow behaviour of erythrocytes through microchannels simulating human blood capillaries
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DOI：
10.1006/mvre.1997.2014
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发表时间：
1997-05-01
影响因子：
3.1
通讯作者：
Rampling, MW
中科院分区：
文献类型：
--
作者：
Sutton, N;Tracey, MC;Rampling, MW
一种新的仪器已经开发出来，以研究红细胞的微观流变学，因为他们现在通过类似于人类毛细血管的尺寸通道。这些通道是使用微工程技术在硅衬底上制造的。采用精确定义的生理驱动压力和温度，同时精确的实时图像处理允许在运输过程中连续监测单个细胞。该仪器表征了ca样品中的每个细胞。1000的体积和在其通过通道期间的速度分布。体积/速度空间中数据的独特表示为血液的微观流变行为提供了新的见解。图像处理和随后的数据分析使系统能够拒绝异常事件，例如多个细胞转变，从而确保所得数据的完整性。通过采用微流体流动通道的阵列，我们可以在一个阵列内集成许多不同但精确且高度可再现的通道尺寸和几何形状，从而允许对一个样品进行多个同时的等压测量。作为该系统的性能的说明，体积/速度的数据集记录在微流体装置，包括多个通道的100 μ m的长度和个别的宽度范围在3.0和4.0 μ m之间。(C)北京：科学出版社.
A novel instrument has been developed to study the microrheology of erythrocytes as they now through channels of dimensions similar to human blood capillaries. The channels are produced in silicon substrates using microengineering technology. Accurately defined, physiological driving pressures and temperatures are employed whilst precise, real-time image processing allows individual cells to be monitored continuously during their transit. The instrument characterises each cell in a sample of ca. 1000 in terms of its volume and how velocity profile during its transit through a channel. The unique representation of the data in volume/velocity space provides new insights into the microrheological behaviour of blood. The image processing and subsequent data analysis enable the system to reject anomalous events such as multiple cell transits, thereby ensuring integrity of the resulting data. By employing an array of microfluidic now channels we can integrate a number of different but precise and highly reproducible channel sizes and geometries within one array, thereby allowing multiple, concurrent, isobaric measurements on one sample. As an illustration of the performance of the system, volume/velocity data sets recorded in a microfluidic device incorporating multiple channels of 100 mu m length and individual widths ranging between 3.0 and 4.0 mu m are presented. (C) 1997 Academic Press.