Five-Month Incubation of Viviparous Deep-Water Shark Embryos in Artificial Uterine Fluid
Five-Month Incubation of Viviparous Deep-Water Shark Embryos in Artificial Uterine Fluid
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DOI：
10.3389/fmars.2022.825354
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发表时间：
2022-03-02
影响因子：
3.7
通讯作者：
Sato, Keiichi
中科院分区：
文献类型：
--
作者：
Tomita, Taketeru;Toda, Minoru;Sato, Keiichi
在这项研究中，我们开发了一种用于胎生软骨鱼的子宫外生命支持系统（人工子宫）。使用该系统，我们将两个细尾灯笼鲨 (Etmopterus molleri) 胚胎标本保存了大约 5 个月，这是该物种圈养保存胚胎最长的已发表记录。该系统的特点是使用含尿素的人工孵化液，其盐度和渗透压与鲨鱼血浆大致相当。我们假设这种液体降低了胚胎血浆和环境液体之间的盐度梯度，从而有助于对抗胎生鲨鱼胚胎的低渗透调节能力。然而，孵化出来的个体随后在“人工分娩”后的海水适应过程中死亡。因此，有关整个妊娠期子宫化学动力学的信息对于设计更有效的孵化系统是必要的。这项技术将具有相关性，并可能在未来应用于公共水族馆的保护性繁殖。
In this study, we developed an extra-uterine life-support system (artificial uterus) for viviparous elasmobranchs. Using this system, we maintained two embryonic specimens of the slendertail lantern shark (Etmopterus molleri) for approximately 5 months, the longest published record of the captive maintenance of the embryos of this species. This system is characterized by the use of a urea-containing artificial incubation fluid, of which the salinity and osmotic pressure are approximately equivalent to those of shark blood plasma. We hypothesized that this fluid reduces the salinity gradient between embryonic blood plasma and the environmental fluid, which thereby contributes to countering the low osmoregulatory capacity of viviparous shark embryos. However, the incubated specimens subsequently died during the process of seawater adaptation following "artificial birth." Accordingly, information pertaining to uterine chemical dynamics throughout the gestation period is necessary for the design of a more effective incubation system. This technique will be relevant and may find application in conservation breeding at public aquaria in the future.