The effect of a chromogranin A-derived peptide (CgA4-16) in the writhing nociceptive response induced by acetic acid in rats
The effect of a chromogranin A-derived peptide (CgA4-16) in the writhing nociceptive response induced by acetic acid in rats
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DOI：
10.1016/j.lfs.2004.02.035
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发表时间：
2004-08-27
期刊：
影响因子：
6.1
通讯作者：
Angel, F
中科院分区：
文献类型：
--
作者：
Ghia, JE;Crenner, F;Angel, F
在炎症性（躯体-内脏）疼痛模型上研究了由嗜铬粒蛋白a （CgA）衍生的合成肽（CgA4-16）的内源性伤害效应。炎症介质参与控制储存和释放嗜铬颗粒的肠染色质细胞的活性。以成年雄性Wistar大鼠为研究对象，腹腔注射稀释乙酸（AA）诱导腹部扭动。在CgA4-16和/或AA联合注射前注射药物。虽然单独使用i.p CgA4-16不产生任何影响，但该肽增加了i.p AA引起的腹部收缩次数，并呈剂量相关。为了确定CgA4-16产生预感效应的可能机制，我们对地尔硫卓或吲哚美辛进行了测试。两种物质预处理均可阻断CgA4-16诱导的前觉效应。静脉给药CGRP、P物质（SP）或辣椒素引起剂量相关的腹部扭动。CgA4-16比CGRP或辣椒素早20分钟，增强了CGRP或辣椒素诱导的伤害效应，而不是SP诱导的伤害效应。综上所述，这些数据首次表明cga衍生的肽可能调节炎症性疼痛。(C) 2004爱思唯尔公司版权所有。
The nociceptive effects of i.p administration of a synthetic peptide (CgA4-16) derived from chromogranin A (CgA) were studied on a model of inflammatory (somato-visceral) pain. Inflammatory mediators participate in controlling the activity of enterochromaffin cells that store and release chromogranins. Adult male Wistar rats were injected i.p with diluted acetic acid (AA) to induce abdominal writhes. Pharmacological agents were injected prior to CgA4-16 and/or AA together. While i.p CgA4-16 alone did not produce any effect, the peptide increased the number of abdominal constrictions induced by i.p AA administration in a dose-related manner. To determine the possible mechanisms involved in CgA4-16 produced pronociceptive effect, i.p diltiazem or indomethacin were tested. The pronociceptive effect induced by CgA4-16 was blocked by pretreatment of either substance. I.p administration of CGRP, substance P (SP) or capsaicin evoked dose-related abdominal writhing. CgA4-16, 20 min prior to CGRP or capsaicin, potentiated the nociceptive effects induced by CGRP or capsaicin, but not those induced by SP. Taken together, these data suggest for the first time that a CgA-derived peptide may modulate inflammatory pain. (C) 2004 Elsevier Inc. All rights reserved.