Sex- and Depot-specific Lipolysis Regulation in Human Adipocytes: Interplay between Adrenergic Stimulation and Glucocorticoids
Sex- and Depot-specific Lipolysis Regulation in Human Adipocytes: Interplay between Adrenergic Stimulation and Glucocorticoids
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DOI：
10.1055/s-0028-1087168
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发表时间：
2008-12-01
影响因子：
2.2
通讯作者：
Eriksson, J. W.
中科院分区：
文献类型：
--
作者：
Lundgren, M.;Buren, J.;Eriksson, J. W.
本研究的目的是探讨肾上腺素能刺激、糖皮质激素和胰岛素对皮下和网膜脂肪库中分离的人类脂肪细胞的脂肪分解率的相互作用，并探讨可能的性别差异。对48名接受选择性腹部手术的非糖尿病患者进行了脂肪活检。通过从分离细胞中释放甘油来测量脂解速率，通过免疫印迹法评估参与脂解调节的蛋白质。采集空腹血液样本，分析代谢和炎症变量。在女性中，皮下脂肪细胞中8-溴camp和异丙肾上腺素刺激的脂肪分解率分别比网膜脂肪细胞高2倍和1.5倍，而在男性中，这两种脂肪细胞之间没有差异。地塞米松治疗增加了8-溴- camp刺激女性皮下脂肪分解的能力，但对男性脂肪细胞没有一致的影响。脂肪细胞中的蛋白激酶A、periilipin A和激素敏感脂肪酶含量不受脂肪储备、性别或糖皮质激素治疗的影响。综上所述，儿茶酚胺和糖皮质激素对分离的人脂肪细胞脂肪分解的调节在脂肪组织储存库和性别之间是不同的。这些发现可能与内源性应激激素和脂肪组织功能在内脏脂肪和代谢综合征中的相互作用有关。
The purpose of this investigation was to explore interactions between adrenergic Stimulation, glucocorticoids, and insulin on the lipolytic rate in isolated human adipocytes from subcutaneous and omental fat depots, and to address possible sex differences. Fat biopsies were obtained from 48 nondiabetic subjects undergoing elective abdominal Surgery. Lipolysis rate was measured as glycerol release from isolated cells and proteins involved in lipolysis regulation were assessed by immunoblots. Fasting blood samples were obtained and metabolic and inflammatory, variables were analyzed. In women, the rate of 8-bromo-cAMP- and isoprenaline-stimulated lipolysis was similar to 2- and 1.5-fold higher, respectively, in subcutaneous compared to omental adipocytes, whereas there was no difference between the two depots in men. Dexamethasone treatment increased the ability of 8-bromo-cAMP to stimulate lipolysis in the subcutaneous depot in women, but had no consistent effects in fat cells from men. Protein kinase A, Perilipin A, and hormone sensitive lipase content in adipocytes was not affected by adipose depot, sex, or glucocorticoid treatment. In conclusion, catecholamine and glucocorticoid regulation of lipolysis in isolated human adipocytes differs between adipose tissue depots and also between sexes. These findings may be of relevance for the interaction between endogenous stress hormones and adipose tissue function in visceral adiposity and the metabolic syndrome.