Mitotic proliferation of myoepithelial cells during regeneration of atrophied rat submandibular glands after duct ligation
Mitotic proliferation of myoepithelial cells during regeneration of atrophied rat submandibular glands after duct ligation
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DOI：
10.1111/j.1600-0714.2004.00234.x
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发表时间：
2004-08-01
影响因子：
3.3
通讯作者：
Wakita, M
中科院分区：
文献类型：
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作者：
Takahashi, S;Shinzato, K;Wakita, M
背景：本研究的目的是阐明萎缩后的大鼠颌下腺再生过程中肌上皮细胞是否进行有丝分裂增殖。方法：用金属夹将大鼠右侧颌下腺的排泄管在肺门附近双重结扎，结扎7天（第0天）后将金属夹取出。移除夹子后0至14天，将再生的右颌下腺移除。切除组织进行免疫组化双染，以增殖细胞核抗原（PCNA）为增殖细胞标志物，肌动蛋白为肌上皮细胞标志物，并用透射电镜（TEM）进行检查。 结果：各时间点再生腺体移行腺泡结构、导管和腺泡周围均可见PCNA阳性肌上皮细胞，尤其是肌上皮细胞PCNA标记指数大幅增加。第2天至第4天之间。第4天时通过TEM也鉴定出肌上皮细胞的有丝分裂。结论：这些结果表明，在大鼠颌下腺再生过程中，肌上皮细胞能够进行有丝分裂增殖。
BACKGROUND: The purpose of the present study was to elucidate whether myoepithelial cells proliferate mitotically during regeneration of rat submandibular glands after atrophy.METHODS: The excretory duct of the right submandibular gland of rats was doubly ligated near the hilum with metal clips, which were removed after 7 days of ligation (day 0). The regenerating right submandibular glands were removed from 0 to 14 days after removal of the clips. The removed tissue was examined with immunohistochemical double staining for proliferating cell nuclear antigen (PCNA) as a marker of proliferating cells and actin as a marker of myoepithelial cells, as well as with transmission electron microscopy (TEM).RESULTS: The PCNA-positive myoepithelial cells were observed at the periphery of transitional duct-acinar structures, ducts and acini in the regenerating glands at every time-point, and the PCNA-labeling index of myoepithelial cells increased greatly especially between day 2 and 4. The mitosis of myoepithelial cell was also identified by TEM at day 4.CONCLUSION: These findings suggest that myoepithelial cells are able to proliferate mitotically during regeneration of rat submandibular gland.