The neutrophil, not the tumor - Serum CA 15-3 elevation as a result of granulocyte - Colony-stimulating factor-induced neutrophil MUC1 overexpression and neutrophilia in patients with breast carcinoma receiving adjuvant chemotherapy
The neutrophil, not the tumor - Serum CA 15-3 elevation as a result of granulocyte - Colony-stimulating factor-induced neutrophil MUC1 overexpression and neutrophilia in patients with breast carcinoma receiving adjuvant chemotherapy
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DOI：
10.1002/cncr.20581
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发表时间：
2004-10-15
期刊：
影响因子：
6.2
通讯作者：
Pavlidis, N
中科院分区：
文献类型：
--
作者：
Pentheroudakis, G;Malamou-Mitsi, V;Pavlidis, N
背景资料。接受粒细胞集落刺激因子(G-CSF)辅助化疗的乳腺癌切除患者血清CA15-3水平升高。作者研究了G-CSF诱导的中性粒细胞MUC1表达在这一环境中的作用。22例早期乳腺癌切除患者和6例高度恶性淋巴瘤患者接受了联合或不联合G-CSF支持的化疗周期。给药时，G-CSF每周期5天或10天。治疗过程中对外周血中性粒细胞和骨髓髓系细胞进行免疫细胞化学染色和流式细胞术分析MUC1表位。基线时，血清CA15-3中位数为16U/mL，外周中性粒细胞表达MUC1弱(中位免疫细胞化学评分[ICCS]=40，流式细胞术评分[FCS]=211个抗体分子/中性粒细胞)。接受5天G-CSF支持化疗的患者，CA15-3水平中度升高(中位数=28U/mL；P=0.016)，中性粒细胞绝对数没有增加，而ICC染色中度增加(中位数ICCS=105；P=0.015)。在接受10天G-CSF化疗周期的患者中，血清CA15-3水平较基线水平升高2-4倍，达到异常水平(中位数=47；P
Background. Patients with resected breast carcinoma who received granulocyte-colony-stimulating factor (G-CSF)-supported adjuvant chemotherapy exhibited an increase in their serum CA 15-3 levels. The authors investigated the role of G-CSF-induced neutrophil MUC1 expression in this setting.Methods. Twenty-two patients with resected early breast carcinoma and 6 patients with high-grade lymphoma received chemotherapy cycles with or without G-CSF Support. When given, G-CSF was administered for either 5 or 10 days per cycle. Immunocytochemical staining and flow cytometric analysis of peripheral blood neutrophils and bone marrow myeloid cells for MUC1 epitopes were performed during treatment.Results. At baseline, the median serum CA 15-3 was 16 U/mL, with weak MUC1 expression in peripheral neutrophils (median immunocytochemical score [ICCS]=40, flow cytometric score [FCS]=211 antibody molecules per neutrophil). For patients receiving chemotherapy cycles with 5-day G-CSF support, median CA 15-3 levels increased moderately (median=28 U/mL; P=0.016) and absolute neutrophil counts (ANC) did not increase, whereas ICC staining showed a moderate increase (median ICCS=105; P=0.015). For patients receiving chemotherapy cycles with 10-day G-CSF, serum CA 15-3 levels increased 2-4-fold from baseline levels and reached abnormal levels (median=47; P