NITRIC-OXIDE ATTENUATES LEUKOCYTE-ENDOTHELIAL INTERACTION VIA P-SELECTIN IN SPLANCHNIC ISCHEMIA-REPERFUSION
NITRIC-OXIDE ATTENUATES LEUKOCYTE-ENDOTHELIAL INTERACTION VIA P-SELECTIN IN SPLANCHNIC ISCHEMIA-REPERFUSION
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DOI：
10.1152/ajpgi.1994.267.4.g562
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发表时间：
1994-10-01
影响因子：
4.5
通讯作者：
LEFER, AM
中科院分区：
文献类型：
--
作者：
GAUTHIER, TW;DAVENPECK, KL;LEFER, AM
在活体显微镜下观察外源性一氧化氮(NO)对戊巴比妥钠麻醉大鼠内脏动脉缺血60min再灌流(SAO/R)后白细胞-内皮细胞相互作用的影响。NO供体S-亚硝基-N-乙酰青霉胺(SNAP)在再灌流前10min静脉滴注20mU·kg(-1)·h(-1)，可显著降低白细胞与内皮细胞的相互作用。这表现为毛细血管后小静脉中的白细胞滚动和粘连显著减少。经SNAP处理的SAO/R大鼠血浆游离氨基氮浓度显著低于未经SNAP处理的SAO/R大鼠(P<；0.05)，显示出组织保护作用。免疫组织化学结果显示，再灌注前10min给予SNAP的大鼠SAO/R后，血管内皮细胞P-选择素的表达显著降低(分别为23.0+/-3.2%和54.9+/-12.1%，P均<0.01)。根据这些数据，我们得出结论，外源性NO对缺血-再灌注后白细胞-内皮细胞相互作用的影响至少部分是通过内皮细胞黏附分子P-选择素介导的。
We studied the effects of exogenous nitric oxide (NO) on leukocyte-endothelial interaction after 60 min of splanchnic artery ischemia and 120 min of reperfusion (SAO/R) in pentobarbital sodium-anesthetized rats via intravital microscopy. Treatment with the NO donor S-nitroso-N-acetylpenicillamine (SNAP, 20 mu g/kg bolus followed by infusion at 20 mu g.kg(-1).h(-1)), beginning 10 min before reperfusion, resulted in significantly decreased leukocyte-endothelial interaction. This was manifested by a significant decrease in leukocyte rolling and adherence in the postcapillary venules. Tissue protection was demonstrated by a significantly lower plasma free amino-nitrogen concentration in the SNAP-treated SAO/R rats compared with those receiving NO-depleted SNAP (P < 0.05). Immunohistochemical localization of P-selectin showed significantly decreased P-selectin expression on the venular endothelium after SAO/R in rats given SNAP 10 min before reperfusion (23.0 +/- 3.2% vs. 54.9 +/- 12.1% positive staining, respectively, P < 0.01). From these data, we conclude that the effects of exogenous NO on leukocyte-endothelial interaction after ischemia-reperfusion appear to be at least partially mediated through the endothelial adhesion molecule P-selectin.