Light sensitive short hypocotyl (LSH) confer symbiotic nodule identity in the legume Medicago truncatula
Light sensitive short hypocotyl (LSH) confer symbiotic nodule identity in the legume Medicago truncatula
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DOI：
10.1101/2023.02.12.528179
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发表时间：
2023-02
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通讯作者：
K. Schiessl;T. Lee;M. Orvošová;M. Bueno-Batista;N. Stuer;P. Bailey;K. Mysore;J. Wen;G. Oldroyd
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作者：
K. Schiessl;T. Lee;M. Orvošová;M. Bueno-Batista;N. Stuer;P. Bailey;K. Mysore;J. Wen;G. Oldroyd
豆科植物生长的特殊根瘤在形态和功能上与侧根不同，根瘤细胞内含有有益的固氮细菌，为植物提供氮源。我们以前已经表明，侧根样程序支持结节的启动，但必须有额外的发展计划，赋予结节的身份。在这里，我们表明，两个成员的光敏感短下胚轴（LSH）转录因子家族，主要是已知的定义器官边界和分生组织的复杂性在拍摄，功能作为调节器的结节器官身份。LSH 1/LSH 2在已知的结节调节因子核因子Y-A1和NODROOT 1/2的上游并与其一起起作用。LSH 1/LSH 2功能的主要结果是产生能够容纳固氮细菌的细胞，这是独特的结节特征。我们的结论是，协调招聘预先存在的原基身份程序，在平行的根启动程序，支持侧根和结节之间的分歧。
Legumes grow specialized root nodules that are distinct from lateral roots in morphology and function, with nodules intracellularly hosting beneficial nitrogen-fixing bacteria that provide the plant with a nitrogen source. We have previously shown that a lateral root-like program underpins nodule initiation, but there must be additional developmental programs that confer nodule identity. Here, we show that two members of the LIGHT SENSITIVE SHORT HYPOCOTYL (LSH) transcription factor family, predominantly known to define organ boundaries and meristem complexity in the shoot, function as regulators of nodule organ identity. LSH1/LSH2 function upstream of and together with the known nodule regulators Nuclear Factor Y-A1 and NODULE ROOT1/2. The principal outcome of LSH1/LSH2 function is the production of cells able to accommodate nitrogen-fixing bacteria, the unique nodule feature. We conclude that the coordinate recruitment of a pre-existing primordium identity program, in parallel to a root initiation program, underpins the divergence between lateral roots and nodules.