Preparation and Antifungal Activities of Microcapsules of Neem Extract Used in Populus tomentosa Deteriorated by Three Mold Fungi

Preparation and Antifungal Activities of Microcapsules of Neem Extract Used in Populus tomentosa Deteriorated by Three Mold Fungi
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印楝提取物微胶囊的制备及用于三霉菌腐烂毛白杨的抗真菌活性

DOI:
10.15376/biores.13.4.8373-8384
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发表时间:
2018-09
期刊:
影响因子:
1.5
通讯作者:
王立海
王立海
中科院分区:
材料科学4区
文献类型:
--
作者:
常璐璐;徐国祺;王立海

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用光学显微镜(OM)和扫描电子显微镜(SEM)对印迹提取物(MNE)微胶囊进行了观察。采用琼脂扩散法检测提取物的抗真菌活性。通过全细胞方法将MNE诱导到木材材料中。用光学显微镜测量了微胶囊的直径,用扫描电子显微镜观察了微胶囊在木材中的分布。用MNE、NE和三聚氰胺甲醛树脂(MF)和十二烷基硫酸钠(AMS)的酸性混合物对毛白杨木材进行处理,观察了它们对桔青霉、绿色木霉和黑曲霉的抑菌性能。由微球、1%十二烷基硫酸钠(十二烷基硫酸钠)和10%NH4Cl制得的微囊形状规则,分散性好。琼脂扩散试验表明,印属植物提取物对所有供试真菌均有明显的抑制作用,最适浓度为10%。微囊直径在0.4μm~4μm范围内呈正态分布,微囊在毛白杨导管和表面分布不均匀。MNE处理的木材样品对所研究的所有真菌均有完全抑制作用,MNE处理的样品对3种霉菌的评分均达到5。
In this work, microcapsules of neem extract (MNE) were observed by an optical microscope (OM) and scanning electron microscope (SEM). The antifungal activity of the extract was evaluated by an agar diffusion assay. The MNE were induced into the wood material by a full-cell process. The diameters of the microcapsules were measured by an OM, and the distribution of microcapsules in wood was observed by a SEM. Wood blocks of Populus tomentosa were treated with the MNE, NE, and an acid mixture of melamine formaldehyde resin (MF) and sodium dodecyl sulfate (AMS); their antifungal properties against Penicillium citrinum, Trichoderma viride, and Aspergillus niger were visually assessed. The microcapsules prepared by MF, 1% sodium dodecyl sulfate (SDS), and 10% NH4Cl possessed regular shape and good dispersion. The agar diffusion assay showed that the neem extract had significant inhibition against all tested fungi, and 10% was the optimum concentration. The diameters of the microcapsules were normally distributed in a range of 0.4 μm to 4 μm, and the microcapsules were unevenly distributed in the vessels and surface of Populus tomentosa. Wood specimens treated with MNE observed complete inhibition to all studied fungi, and the mark grades of specimens treated with MNE against three mold fungi all reached 5.
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