Identification of a Novel Second-Generation Circulating Recombinant Form (CRF48_01B) in Malaysia: A Descendant of the Previously Identified CRF33_01B
Identification of a Novel Second-Generation Circulating Recombinant Form (CRF48_01B) in Malaysia: A Descendant of the Previously Identified CRF33_01B
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DOI：
10.1097/qai.0b013e3181d82ce5
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发表时间：
2010-06-01
影响因子：
3.6
通讯作者：
Takebe, Yutaka
中科院分区：
文献类型：
--
作者：
Li, Yue;Tee, Kok Keng;Takebe, Yutaka
2007 年在马来西亚半岛东部的注射吸毒者中进行的一项分子流行病学调查发现了一组位于任何已知 HIV-1 基因型之外的序列 (n = 3)。对这些来自没有可识别连锁的个体的菌株的近全长核苷酸序列的分析表明，它们具有由 CRF01_AE 和亚型 B' 组成的相同亚型结构，与任何已知的循环重组形式 (CRF) 不同。这种新型 CRF 被指定为 CRF48_01B，与先前在吉隆坡发现的 CRF33_01B 密切相关。对多个CRF48_01B基因组区域的系统发育分析表明，CRF48_01B在CRF33_01B内形成单系簇，表明该新重组体很可能是CRF33_01B的后代。因此，CRF48_01B 代表了“第二代”CRF 的第一个例子，它是通过与预先存在的 CRF 进行额外交叉而生成的。贝叶斯分子钟分析证实了这些结果，表明 CRF48_01B 出现的时间与 2001 年类似，大约与 CRF33_01B 出现后 8 年类似。
A molecular epidemiological investigation conducted among injecting drug users in eastern Peninsular Malaysia in 2007 identified a cluster of sequences (n = 3) located outside any known HIV-1 genotype. Analyses of near full-length nucleotide sequences of these strains from individuals with no recognizable linkage revealed that they have an identical subtype structure comprised of CRF01_AE and subtype B', distinct from any known circulating recombinant forms (CRFs). This novel CRF, designated CRF48_01B, is closely related to CRF33_01B, previously identified in Kuala Lumpur. Phylogenetic analysis of multiple CRF48_01B genome regions showed that CRF48_01B forms a monophyletic cluster within CRF33_01B, suggesting that this new recombinant is very likely a descendant of CRF33_01B. CRF48_01B thus represents one of the first examples of a "second-generation'' CRF, generated by additional crossover with pre-existing CRFs. Corroborating these results, Bayesian molecular clock analyses indicated that CRF48_01B emerged in similar to 2001, approximately similar to 8 years after the emergence of CRF33_01B.