Myogenic repressor I-mfa interferes with the function of Zic family proteins
Myogenic repressor I-mfa interferes with the function of Zic family proteins
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DOI：
10.1016/j.bbrc.2004.05.158
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发表时间：
2004-07-16
影响因子：
3.1
通讯作者：
Aruga, J
中科院分区：
文献类型：
--
作者：
Mizugishi, K;Hatayama, M;Aruga, J
属于Zic家族的锌指蛋白控制几个发育过程，如中轴骨架的图案化。在这里，我们绘制了Zic 2蛋白的转录调控结构域，并确定了一个蛋白质，它特异性地结合到其中之一。在作图实验中，一个氨基末端区域被确定为转录调控结构域。对与Zic 2的氨基末端结构域结合的蛋白质的搜索揭示，MyoD家族抑制剂（I-mfa）蛋白可以与氨基末端结构域物理地相互作用，该抑制剂已被鉴定为肌源性螺旋-环-螺旋类转录因子的阻遏物。当Zic 1 -3和I-mfa蛋白在培养的细胞中共表达时，Zic蛋白的核输入被抑制。因此，I-mfa抑制了培养细胞中Zic蛋白的转录激活。这些结果表明，Zic和I-mfa蛋白之间的物理和功能的相互作用可以在脊椎动物的发育中发挥作用。(C)2004年爱思唯尔公司All rights reserved.
Zinc finger proteins belonging to the Zic family control several developmental processes such as patterning of the axial skeleton. Here we mapped the transcriptional regulatory domains in Zic2 protein and identified a protein which specifically binds to one of them. In the mapping experiments, an amino-terminal region was identified as transcriptional regulatory domains. A search for proteins binding to the amino terminal domain of Zic2 revealed that inhibitor of MyoD family (I-mfa) protein, which has been identified as a repressor of myogenic helix-loop-helix class transcription factors, can physically interact with the amino terminal domain. When Zic1-3 and I-mfa proteins were co-expressed in cultured cells, nuclear import of the Zic proteins was inhibited. Consequently, I-mfa inhibited transcriptional activation by the Zic proteins in cultured cells. These results suggest that the physical and functional interaction between Zic and I-mfa proteins can play a role in the vertebrate development. (C) 2004 Elsevier Inc. All rights reserved.